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Premade Human and Mouse Antibody Libraries

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HuScL-1(R), HuScL-2(R), HuScL-3(R) and HuScL-4(R) Human Single Chain (scFv) Antibody Libraries are natural or synthetic libraries of a diversity of over 2.0×109. Recently, we have created a next-generation, fully-human, yeast display scFv library - HuScL-YDP. We also have a HuFabL(R) Human Fab Antibody Library of a size of 2.0×109. Besides human antibody libraries, we have a MuScL(R) Murine Single Chain Antibody Library and a MuFabL(R) Murine Fab Antibody Library.

HuScL-1(R): Human Single Chain (scFv) Antibody Library

  1. Phagemid phage display system
  2. Mono-display by pIX fusion
  3. scFv format
  4. Construction strategy: VH and VL genes were fully synthesized; mixture of 18 sub-libraries
  5. Capacity: 1.35×1010
  6. Proliferation in TG1 cells
  7. Helper phage: M13K07

HuScL-2(R): Human Single Chain (scFv) Antibody Library

  1. Phagemid phage display system
  2. Mono-display by pIX fusion
  3. scFv format
  4. Construction strategy: VH and VL genes were semi-synthesized
  5. Capacity: 1.42×109
  6. Proliferation in TG1 cells
  7. Helper phage: M13K07

HuScL-3(R): Human Single Chain (scFv) Antibody Library

  1. Phagemid phage display system
  2. M13 pIII fusion containing short His6 and V5 detection tags
  3. VH-VL format scFv library
  4. Collections of human antibody genes from non-immunized donors
  5. VL/VH chain recombination through an integrated Cre/lox system
  6. Theoretic capacity: 1.0x1016
  7. Proliferation in TG1 cells
  8. Production of soluble antibody fragments in HB2151
  9. Helper phage M13KO7

HuScL-4(R): Human Single Chain (scFv) Antibody Library

  1. Phagemid phage display system
  2. M13 pIII fusion containing short c-Myc detection tag
  3. VH-VL format scFv library
  4. Seven heavy chain frameworks and seven light chain frameworks representing the structural diversity of human antibody repertoire
  5. Complete randomization in "HCDR3" and "LCDR3"
  6. Capacity: 1.5x1016

HuScL-YDP: Yeast Display Human scFv Antibody Library(New)

  1. Yeast display system
  2. Fully-human, scFv library for de novo discovery of therapeutically relevant antibodies
  3. Based on 2 liter of blood from approximately 20 donors
  4. Based on UltraffTM Yeast Display Technology, can be screened with flow cytometric or magnetic bead based methods
  5. Capacity: 1.5x109

HuFabL(R): Human Fab Antibody Library

  1. Phagemid phage display system
  2. M13 gIII fusion containing short His6 and c-Myc detection tag
  3. Fab format
  4. Collections of human antibody genes from non-immunized donors
  5. 2.0x109
  6. Proliferation in XL1-blue cells
  7. Production of soluble antibody fragments in TOP10F'
  8. Helper phage VCSM13

MuScL(R): Murine Single Chain Antibody Library

  1. Phagemid phage display system
  2. M13 pIII fusion
  3. scFv format
  4. Collections of antibody genes from none immunized mice
  5. Capacity: 8.0×108

MuFabL(R): Murine Fab Antibody Library

  1. Phagemid phage display system
  2. M13 gIII fusion
  3. Fab format
  4. Collections of antibody genes from naive mice
  5. Capacity: 6.0x109

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