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IF-Positive Hybridoma Screening

Immunofluorescence (IF) microscopy is generally used by researchers to assess both the localization and endogenous expression levels of proteins of interest. Armed with rich experience, Creative Biolabs provides IF-guaranteed antibody development by our innovative hybridoma preparation and validation platform.

Immunofluorescence (IF)

Fluorescent-labeled antibodies are the crucial reagents of IF methods, a specific type of immunostaining approach. In its essence, IF is similar to immunohistochemistry (IHC)/immunocytochemistry (ICC), therefore it can be used to detect and visualize a protein of interest on fixed or frozen tissues or particular cell types. The difference lies in how the samples are visualized. In detail, IHC/ICC assays rely on an enzymatic reporter and a substrate to produce a color change. IF assays require only a fluorescent label and the proper lasers to produce a signal. In both cases, multiplexing antigen detection is possible provided that the fluorochromes or enzymatic reporters emit non-overlapping signals.

Developing antibodies for IF use can be especially challenging since these reagents need to be highly specific to the target and, at the same time, cause minimal background noise. Moreover, when different antibody conjugates are used in the same assay, it is even more important to validate the panel or cocktail of the different antibodies to ensure the data generated by IF experiments are easily interpreted.

Our functional application validation ensures the candidate antibody works in IF application. If you are looking for custom function guaranteed antibody development service, please feel free to contact us.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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