WB&IP-Positive Hybridoma Screening

Custom hybridoma development services at Creative Biolabs provide a one-stop shop for high-quality antibody production. Through our years of experience generating custom antibodies for global customers, we pride ourselves in producing high specificity monoclonal and polyclonal antibodies for desired applications for basic research use. Our process of hybridoma generation includes a broad offer of application guaranteed packages ensuring your antibodies keep their high affinity, specificity, and stability in your unique assay format and conditions.

WB & IP

Western blot (WB) is a widely used method to separate and detect proteins. It is typically performed in denaturing conditions meaning that the secondary and tertiary structures of the desired marker are lost. The development of antibodies for WB use needs to take this loss of native structure into account. More importantly, it remains important to validate antibodies for WB in denaturing conditions to ensure they detect only the marker of interest.

Immunoprecipitation (IP) is a popular technique to capture and concentrate proteins from complex mixtures. It allows the enrichment of specific markers, which is particularly useful when dealing with low-abundance proteins. Moreover, IP can be used to study the interaction of the target protein with other molecules as these complexes tend to co-precipitate. Besides, IP is often used in tandem with mass spectrometry providing a powerful method for measuring and identifying low-abundance proteins in complex samples. Thus, antibodies intended for IP use need to be carefully validated in the test conditions to ensure only the target protein or complex is efficiently recovered from specific samples.

Fig.1 Hybridoma development services workflow.

Service Profile

Achieve your unique project goals by choosing the most robust and flexible solution for diagnostic antibody development! For therapeutic research use, our hybridoma development service is tailored to maximize antibody affinity towards clinically relevant targets. For diagnostic research use, our hybridoma development process takes into account the antibody's final application. All diagnostic antibodies generated in our hybridoma platform are optimized and validated in the application of your choice considering assay format and conditions, sample composition, and processing protocol. In this way, we ensure all antibodies keep the highest level of performance. Figure 1 shows our hybridoma development service content. In general, hybridomas will be screened firstly by ELISA for at least 2 rounds. Subsequently, WB and IP assays are done directly in the target application. Selected clones are subcloned for further antibody production.

The unique advantages of using hybridoma generation

A robust and mature approach with a proven track record for the cost-effective generation of high-quality antibodies.
Higher stability, which makes them ideal when testing the presence of specific markers even in harsh and complex environments.
Highly efficient in vivo affinity maturation.

If you are looking for WB&IP-positive hybridoma development service, please feel free to contact us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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