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Circular RNA Sequencing (CircRNA-Seq) Service

Introduction Workflow Samples Depth Features Advantages Q&As Resource

With the state-of-art instruments and professional staffs, Creative Biolabs has over a decade of working experience in next-generation sequencing (NGS). Based on our powerful SuPrecision™ platform, Creative Biolabs is proud to offer you with the circRNA sequence information with single-base resolution. We can provide the most comprehensive bioinformatics analysis, including circRNA identification and annotation, known circRNA, novel circRNA prediction, origin analysis of circRNA and target prediction. We are pleased to use our extensive experience and advanced platform to offer the best service to satisfy each demand from our customers.

The Introduction of CircRNA

CircRNA, an addition to the growing list of types of non-coding RNA, is a large group of RNA family extensively exists in cells and tissues. CircRNAs can arise from exons (exonic circRNA) or introns (intronic circRNA). Although the existence of circular transcripts has been known for at least 20 years, such molecules were long considered molecular flukes—artifacts of aberrant RNA splicing or specific to a few pathogens, such as the Hepatitis δ virus 3 and some plant viroids. However, potential functions in the regulation of gene expression are emerging for both exonic and intronic circRNAs. Recent work has revealed large numbers of circRNAs that are endogenous to mammalian cells, and many of these are abundant and stable. Circular RNAs, unlike miRNAs and other small RNAs, are not easily separated from other RNA species by size or electrophoretic mobility. Commonly used molecular techniques require amplification and/or fragmentation destroy circularity, however, because of the lack of free 3’ or 5’ end, circRNAs cannot be found by molecular techniques that rely on a polyadenylated free RNA end. The advent of high-throughput NGS provides a promising way to view circRNAs across different samples, especially in cancers.

WorkFlow of CircRNA-seq at Creative Biolabs

Total RNA
Removal
of
rRNA
Strand-specific library preparation
Sequencing
Data analysis

Sample Preparation:

RNA sample concentration ≥100 ng/μL
Total RNA sample amount ≥10 μg
Purity: OD260/280 = 1.8~2.2, no degradation, no DNA contamination

Recommended Depth

≥ 10 Gbase

Key Features of CircRNA-seq Service Include but Are Not Limited to:

Detect novel and rare transcripts and transcript variants, such as single nucleotide mutations (SNP)
Predict circRNA targets
Localization of circRNAs on genome
Transcriptome-wide – investigation of the known circRNA data and novel circRNA at the same time

Key Advantages of CircRNA-seq Service at Creative Biolabs Include but Are Not Limited to:

Comprehensive spectrum of species
State-of-art NGS technology
Comprehensive bioinformatics analysis
Low false positive
Cost-effective

Enriched experience associated with state-of-the-art facilities, Creative Biolabs has long-term devoted to the development and application of NGS technology. We are pleased to offer circRNA-seq and bioinformatics analysis service with the best quality and the most competitive price.

Please contact us for more information and a detailed quote.

Q&As

Q: What advantages does CircRNA-Seq offer over traditional RNA sequencing?

A: CircRNA-Seq specifically targets circular RNAs, providing a focused and detailed analysis of these molecules. Traditional RNA sequencing may miss or underrepresent circRNAs, making CircRNA-Seq essential for studying these unique RNAs comprehensively.

Q: Can CircRNA-Seq be applied to all types of cancer?

A: Yes, CircRNA-Seq can be applied to various types of cancer. It is particularly useful in cancers where circRNAs are suspected to play significant roles, such as breast cancer, liver cancer, and glioblastoma.

Q: What role does bioinformatics play in CircRNA-Seq?

A: Bioinformatics is essential for processing the large amounts of data generated by CircRNA-Seq. It involves aligning sequences, identifying circRNAs, and interpreting their potential roles in cancer biology. Advanced algorithms and software tools are crucial for accurate analysis.

Q: How does CircRNA-Seq integrate with other sequencing technologies?

A: Integrating CircRNA-Seq with other sequencing technologies, such as RNA-Seq and DNA sequencing, provides a comprehensive view of the genetic and epigenetic landscape of cancer. This multi-omics approach enhances the understanding of complex regulatory networks in cancer cells.

Q: How can circRNA-Seq be integrated into routine cancer research workflows?

A: CircRNA-Seq can be integrated into cancer research workflows by combining it with other molecular techniques, such as RNA-Seq and proteomics. This integration provides a comprehensive understanding of cancer biology and enhances the discovery of new biomarkers and therapeutic targets.