Customer Review
High protein yield
The long protein synthesis time ensures an abundant protein yield.
2023.1.06
Q&As
What are the important steps for successful protein expression and purification using a two-step in vitro human cell-free expression system?
1) successful amplification and cloning of genes into pT7CFE-CHis plasmid vector; 2) transcribing RNA at 30oC; 3) translation of protein at 30 °C in the presence of RNAse inhibitor; 4) developing the affinity based purification protocol using resin beads coated with Ni and 5) analyzing results on western blot using polyclonal and monoclonal antibodies.
2023.01.10
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