Category:
Non-Human Primate (NHP) related Cell Overexpression Lysate Product
Tag: Cynomolgus Monkey,Cell Overexpression Lysate
Tag: Cynomolgus Monkey,Cell Overexpression Lysate
Cynomolgus Monkey CD58/LFA-3 HEK293 Cell Overexpression Lysate (NHFF-0524-HX610)
Product Overview
Detailed Product Information
Technical Specifications
Storage and Shipping
Downloadable Resources
Customer Reviews
Cynomolgus monkey CD58/LFA-3 HEK293 cell overexpression lysate is prepared from the homogenate of CD58/LFA-3 overexpression HEK293 cells and is mainly applied in Western blot experiments as a positive control. Creative Biolabs provides these cell lysates in highly consistent batches and ensures your research results keep good reproducibility.
Source:
From healthy Cynomolgus monkey
Host:
HEK293 cells
Calculated MW:
23 kDa
Apparent MW:
38 - 42 kDa
Method of Preparation:
This cell overexpression lysate was meticulously collected by a series of steps including the cell homogenization in cold optimized RIPA lysis reagent with multiple protease inhibitors, cell debris centrifugation, protein concentration determination by Bradford assay, lysate boiling in 1 x SDS loading buffer containing 5% b-mercaptoethanol, and lysate lyophilization.
Lysis Reagent:
An optimized RIPA lysis reagent is used in the preparation of this product. The specific ingredients are as follows: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, and 1mM PMSF.
Attention before Use:
Centrifuge briefly before use, then dissolve the pellet in 200μl pure water and boil for about 3 minutes.
DNA Sequence:
Met1-Arg215
Tag:
His
Quality Control:
The protein quality is verified by Coomassie blue staining after protein purification.
Storage:
Usually stored at 4°C. Once re-dissolution, please aliquot this lysate into smaller sizes and transfer them into a -80°C refrigerator.
Shipping:
Ambient temperature or ice pack.
- Cynomolgus Monkey CD58/LFA-3 HEK293 Cell Overexpression Lysate (NHFF-0524-HX610) Data Sheet: Detailed information. datasheet
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Your research is unique, and we understand that standard products may not always fit. Our team of experts specializes in custom NHP sample collections. We can design a tailored protocol to meet your specific research requirements, including samples with unique anticoagulant types, customized volumes, or from specific disease models.
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For research use only. Not intended for any clinical use.
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