Category:
Non-Human Primate (NHP) related Cell Overexpression Lysate Product
Tag: Cynomolgus Monkey,Cell Overexpression Lysate
Tag: Cynomolgus Monkey,Cell Overexpression Lysate
Cynomolgus Monkey FGL1 HEK293 Cell Overexpression Lysate (NHFF-0524-HX651)
Product Overview
Detailed Product Information
Technical Specifications
Storage and Shipping
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Specific protein cell overexpression lysate is routinely used as a positive control in Western blot assays. At Creative Biolabs, we constructed the Cynomolgus monkey FGL1 overexpression cell lysate through the manipulation of HEK293 cells. These products with high protein purity will become helpful tools for your immunoblotting experiments.
Source:
From healthy Cynomolgus monkey
Host:
HEK293 cells
Calculated MW:
56.94 kDa
Method of Preparation:
This cell overexpression lysate was meticulously collected by a series of steps including the cell homogenization in cold optimized RIPA lysis reagent with multiple protease inhibitors, cell debris centrifugation, protein concentration determination by Bradford assay, lysate boiling in 1 x SDS loading buffer containing 5% b-mercaptoethanol, and lysate lyophilization.
Lysis Reagent:
An optimized RIPA lysis reagent is used in the preparation of this product. The specific ingredients are as follows: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, and 1mM PMSF.
Attention before Use:
Centrifuge briefly before use, then dissolve the pellet in 200μl pure water and boil for about 3 minutes.
DNA Sequence:
Met37-Ile348
Tag:
Fc
Quality Control:
The protein quality is verified by Coomassie blue staining after protein purification.
Storage:
Usually stored at 4°C. Once re-dissolution, please aliquot this lysate into smaller sizes and transfer them into a -80°C refrigerator.
Shipping:
Ambient temperature or ice pack.
- Cynomolgus Monkey FGL1 HEK293 Cell Overexpression Lysate (NHFF-0524-HX651) Data Sheet: Detailed information. datasheet
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Your research is unique, and we understand that standard products may not always fit. Our team of experts specializes in custom NHP sample collections. We can design a tailored protocol to meet your specific research requirements, including samples with unique anticoagulant types, customized volumes, or from specific disease models.
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For research use only. Not intended for any clinical use.
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