Description
The Organoid Viability Assay Pack is a fluorescence-based kit for determining cell viability in 3D organoids. Metabolic reductases in live cells convert a weak blue dye into a strong red fluorescent signal, proportional to the live cell count. This "add-and-read" assay requires no washing or lysis, is non-toxic, and enables continuous monitoring.
Organ/Tissue Related
General
Species Reactivity
Universal
Application
Viability Assay
Synonyms
Organoid Viability Assay Kit; Cell Viability Detection Kit for Organoids; Fluorescence-Based Organoid Viability Assay; Live/Dead Assay Kit for Organoids; Organoid Health Assessment Kit
Shipping
Shipping with dry ice
Storage
Store at -20°C for 2 years. Protect from light and minimize freeze-thaw cycles.
Precautions
1. Sterile product; handle aseptically. Aliquot to prevent contamination. 2. Optimize cell density (100-100,000 cells/well for 96-well plates) for best sensitivity. 3. Avoid direct sunlight during assay; ambient lab light is acceptable. 4. Adjust incubation time based on cell count. Measure fluorescence from the bottom to avoid interference. 5. Research use only. 6. Wear lab coat and gloves.
