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Magic™ Cell-free Protein Expression in Rabbit Reticulocyte Lysate

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Creative Biolabs is a long-term expert in the field of membrane protein production. After years of dedication, we have successfully built and optimized a versatile cell-free protein synthesis platform which proved to be very effective and powerful for many difficult membrane protein targets. Our scientists are experienced in tailoring the most reliable and cost-effective plans for our global clients to facilitate their diverse research programs.

Cell-free Rabbit Reticulocyte Lysate (RRL) used for protein expression is generated from lysed reticulocytes isolated from rabbits treated with phenylhydrazine. The lysed reticulocytes are pre-treated with micrococcal nuclease to remove endogenous mRNA. During the late 1950s and early 1960s, it was first proved that radioactive amino acids could be incorporated into hemoglobin in cell‑free rabbit reticulocyte lysate. After that RRL has been used to elucidate the highly complex events that encompass translation process. In the 1970s, researchers discovered that RRL could be manipulated for direct protein synthesis exogenously from mRNA. Later on, further supplemented by T3, T7, or SP6 RNA polymerases and many other components, RRL has become a novel cell-free protein expression system and successfully generated a variety of proteins. This system is now widely employed to elucidate the mechanisms of mammalian post-translational modifications, translation, cotranslational modifications, and translocation of proteins. Particularly, it can also be incorporated in microarray, display or in vitro expression cloning (IVEC) technologies for protein evolution, interaction, selection studies. Taken together, rabbit reticulocyte lysate system makes an attractive tool for structural biology, functional genomics, drug screening, and vaccine production.

Mempro™ Cell-Free Protein Production in Rabbit Reticulocyte Figure 1. The strategy of in vitro expression cloning. (The role of cell-free rabbit reticulocyte expression systems in functional proteomics, 2007)

Compared with conventional cell-based/cell-free protein synthesis platforms, RRL system exerts multiple superior features including:


Creative Biolabs has years of experience in the field of cell-free protein expression. Alternative cell-free approaches are also available upon request:


Our scientists take pride in earning great reputation by successfully accomplishing various challenging projects. We are always eager to work with you step by step to meet each specific requirement, and it’s our promise to help worldwide customers with their valuable projects using our professional services. Please feel free to inquire us for more information.

References

  1. Arduengo M, Schenborn E, Hurst R (2007) The role of cell-free rabbit reticulocyte expression systems in functional proteomics. Cell-Free Expression. Austin, TX: Landes Bioscience, 2007, 1-18.
  2. Alkhalfioui F, Logez C, Bornert O, Wagner R (2011) Production of Membrane Proteins - Strategies for Expression and Isolation (Robinson AS, ed), Wiley-VCH: 1.



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