PTK2 Polyclonal Antibody (NHP-AB438)

• This product is an antibody that was generated by immunizing rabbit against a peptide located in the kinase domain of PTK2 (Human). It is used only in in vitro assays.

Detailed Product Description

• Introduction: PTK2 is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. PTK2 plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of PTK2, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion. PTK2 has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased PTK2 tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with PTK2 in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with PTK2 is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated PTK2, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the PTK2 gene, but the full-length natures of only three of them have been determined.
• Conjugate: None
• Applications: IHC

Technical Specifications

• Host Species: Rabbit
• Species Reactivity: Bat; Bovine; Chicken; Dog; Gibbon; Gorilla; Hamster; Human; Monkey; Mouse; Rat
• Immunogen: A peptide located in the kinase domain of PTK2 (Human)

Product Property

• Purification: Immunoaffinity Chromatography
• Format: Liquid
• Concentration: 1 mg/mL
• Buffer: PBS, 0.1% sodium azide
• Storage: Short term: 4˚C. Long term: Store at -20˚C.

Target Information

• Clonality: Polyclonal
• Alternative Names: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal adhesion kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2
• Entrez Gene ID: 5747