Custom sdAb Library Generation Services
As a pioneer and undisputed global leader in the field of sdAb development, Creative Biolabs is professional in applying our custom strategies to satisfy various sdAb library demands. With our extensive experience and advanced library construction platform, we are therefore confident in offering custom sdAb library services to meet our customers’ different objectives and guarantee the most satisfactory results for our customers all over the world.
Custom sdAb Library at Creative Biolabs
The field of sdAb has rapidly progressed during the last two decades, mainly because of the interest in their human therapeutic and diagnostic applications. To identify practical sdAbs, it is essential to start from a high-quality gene bank of sdAbs, dedicated to the final application and with maximal diversity. Currently, our seasoned scientists have set up a series of strategies that can assist in generating custom sdAb libraries with the highest efficiency and best quality. Our powerful strategies that can help our customers include:
Naïve sdAb Library
- No animal immunization
- Larger library size
- Explicitly unbiased nature of the sdAb repertoires
- Capable of generating sdAbs against any specific targets
Immune sdAb Library
- Immunization
- Only require a handful of materials
- Somatic maturation in vivo
- Highly enrichment of target specific sdAbs with high affinity
Synthetic sdAb Library
- Various design strategies are available
- High diversity and utility
- Capable of generating sdAbs against any specific targets
- Extensively used in the industry
First of all, retrieval of sdAbs from an immune library is a priority consideration. Creative Biolabs has unparalleled capabilities for the construction of VHH or VNAR based sdAb libraries through immunized camel, llama, alpaca, or shark in terms of our advanced technology. The immune sdAb library is unique as it gives access to the intact, affinity-matured, antigen-binding fragments. Somatic maturation in lymphocytes of immunized animals will give sdAb libraries a more specific and higher affinity to antigens of interest. Among all species of the immune library, llama and alpaca are often used because they have a smaller size in reality (easy to raise and breed) and these animals are commonly found in European and American markets. sdAb from these animals is proved best-suitable for the therapeutic purpose. In contrast, camel serum has a higher content of sdAbs, the antibody germline is more abundant, and sdAbs from camel have additional disulfide bonds; these data demonstrate that the camel library may be more suitable for applications that require more stable sdAbs, such as diagnostic affinity reagents.
Alternatively, the suitable naïve sdAb library and even synthetic sdAb library are the available solutions to identify antigen binders in cases where difficulties to immunize are encountered, lack of antigen, low immunogenic, or toxic antigen. Although naïve libraries and synthetic sdAb libraries require much larger libraries to retrieve specific binders of high affinity, such diversified sdAb libraries enabled the selection of Ab fragments specific to virtually any target.
Equipped with advanced platforms and powerful technologies, Creative Biolabs is proud to tailor and conduct the best-fit proposal to meet our customers' specific sdAb library requirements. Contact us today to learn how we can help you continue to generate a custom sdAb library.
Case Study
All services are provided for research purposes only. Our services cannot be used for clinical or therapeutic applications.
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