AOC for Proximity Extension Assay (PEA)

Antibody-oligonucleotide conjugates (AOCs) represent new technology and have been used in different applications such as imaging, detection, and therapeutics. Creative Biolabs is a recognized leader of high-quality bioconjugation services at an outstanding high success rate. We provide a full set of antibody-oligonucleotide conjugates (AOCs) development services to meet your special needs. The bioconjugates manufactured by us will be stable and effective, which can be successfully applied in various areas such as proximity extension assay (PEA).

AOC Used for Proximity Extension Assay (PEA) Application

PEA is a direct one-step protein quantification method. It uses a pair of DNA oligonucleotides to connect with the target molecule of the antibody. It needs polyclonal or two monoclonal antibodies (mAbs) to bind closely to the target epitope to form a DNA duplex, which is quantified by real-time PCR.

AOC can be used for the PEA and it is based on the use of two AOC probes, one of which possesses 3′-linked double-stranded DNA, containing a short overhang at its 5′-end. This overhang is used to be complementary to the 5′-linked single-stranded DNA bound to the partner probe. During incubation with mutually recognized samples, hybridization of the overhanging fragments becomes advantageous and the resulting hybridization of double-stranded DNA can be extended by DNA polymerase. This result in a full-length amplicon that can be detected and quantified by qPCR.

Later, this method was optimized so that the double-stranded DNA was replaced by a single strand ON containing complementary sites for direct hybridization with the other ONs, which allowed the extension through the hyper-thermostable DNA polymerase. Furthermore, in multiplex assays, the method can be improved by adding a step of primer digestion after amplification. PEA mainly depends on enzymes, which makes this method relatively expensive and requires special handling and storage. Thus, to overcome this limitation, AOC proximity binding has been combined with polymerization reaction to generate enzyme-free amplification variant of these techniques.

Fig.1 Mode of action of PEA probes for protein detection. (Dovgan, 2019)

Featured AOC Development Services at Creative Biolabs

Supported by a team of scientists and experts in the field of antibody development and bioconjugation, Creative Biolabs offers fully integrated AOCs development services for worldwide customers. With extensive experience and advanced technology platform, our conjugation services will be provided in the most high-quality and cost-effective way. We believe our services will help you to make your project a success.

Features of Our Services

• Performed by experienced scientists
• First-class conjugation services with full of technical support
• Fast turnaround time with professional service
• Effective and cost-effective

If you are interested in the services and want to know more detailed information, please feel free to contact us and get free consultation and quote. We will get in touch with you as soon as possible and help you design an appropriate protocol to meet your specific requirements.

Reference

1. Dovgan, I.; et al. Antibody-oligonucleotide conjugates as therapeutic, imaging, and detection agents. Bioconjugate chemistry. 2019, 30(10): 2483-2501.

For Research Use Only. NOT FOR CLINICAL USE.