MultiProbe

Recent advances in biochemistry and biomaterials enable the continuous development of new materials and their applications to diverse fields including high-resolution imagery, medicine, and diagnosis. Among the numerous new materials, bio-conjugates, generated via the bio-conjugation process, is a big and important family. Bio-conjugation, a strategy to form stable linkage between two components, is crucial for the properties of the final products. The most common bio-conjugations are achieved via the coupling a protein, specifically an antibody or antibody derivative, with different substances to yield a series of antibody-based bio-conjugates. Common substances used in antibody-based bio-conjugates generation include fluorophores, radioisotope labels, nucleic acids, polysaccharides, natural or synthetic toxins, as well as a series of synthetic particles such as quantum dots, gold nanoparticles, magnetic nanoparticles, liposomes and polymer micelles. Generally, the functional moieties on the side chains of Lys, Cys, Tyr residues or at the N-/C-termini of antibodies or antibody derivatives can be used for bio-conjugation reactions to proper functional groups on the conjugating substances. In some cases, a small linker (e.g., a bifunctional polyethylene glycol or chelator) is introduced as the bridge between the antibody and the conjugating substance. The antibody-based bio-conjugates generated via proper conjugation strategies maintain the functionalities of both components, enabling their applications as probes for diverse theranostic applications. For instance, directed by the antibody in the probe, antibody-superparamagnetic iron oxide nanoparticle probes can specifically accumulate at the tumor site and serve as contrast agents for high-resolution magnetic resonance imaging (MRI). On the other hand, antibody-based bioconjugates, with negligible distribution at non-targeted/normal sites, can overcome some fatal drawbacks of free probes, e.g., toxicity and disruptive effects to normal tissues, or unwanted noises in the diagnostic imagery.

A simplified illustration of bio-conjugation strategies: the reaction can occur either directly between the antibody and probe moieties (up right), or via a linker as the bridge between the antibody and probe moieties (bottom right).

With the development of biochemical technologies, a broad range of materials are suitable for the generation of innovated antibody-based bio-conjugates, leading to the explosion of the number of antibody-based probes. Those probes are designed and designated to fulfill the requirements of diverse scientific studies and biomedical applications.

Besides the well-established antibody-based probe systems, Creative Biolabs also customizes antibody-based probes and provides designer probes according to customers’ specific demands in our MultiProbe services. With extensive expertise in antibody development and our comprehensive antibody library, Creative Biolabs has generated a variety of engineered antibodies, including various monoclonal antibodies, recombinant antibodies, bispecific antibodies, Fab fragment antibodies, scFv fragment antibodies, and single domain antibodies, as targeting moieties in antibody-based probes. In terms of the functionality moieties, MultiProbe services at Creative Biolabs are not limited to the production of traditional probes with single functional or single detectable property. MultiProbe services are designated to develop antibody-based probes with multi-detectability or multi-functionalities. For instance, the superparamagnetic iron oxide nanoparticles are modified further with fluorophores and loaded with certain cytotoxins. Upon conjugation with antibodies for certain tumor targets, this antibody-fluorescent iron oxide nanoparticle probe that specifically accumulate at tumor sites can be used as contrast agents for both high-resolution fluorescence imaging and MRI for the diagnosis of cancer. Meanwhile, upon internalization by the tumor cells, the probes can release cytotoxic payloads and kill cancer cells to achieve the anti-cancer therapeutic functionality.

A brief summary of materials that can be bio-conjugated into antibody-based probes for numerous theranostic applications, including high-resolution imagery, payloads delivery, as well as the diagnosis and therapy of diverse disease. The inner circle includes the major antibody based products as targeting moieties in the probe; the outer circle includes a broad range of inorganic and organic complexes as functional moieties in the probe.  Abbreviations: Ab, antibody; BiTE, bispecific T-cell engager; scFv, single-chain variable fragment.

MultiProbe is designated for uniqueness! For customers who have special needs for antibody-based probes, the MultiProbe service platform at Creative Biolabs can provide a wide range of services including the selection of antibody-related targeting ligands and functional moieties, conjugation strategy design, method development, assay validation, as well as scale-up production of highly customized antibody-based probes. In this one-stop-shop of designer antibody-based probes, we are dedicated to make your ideas into reality. Please contact us for more information and a detailed quote.

For Research Use Only. NOT FOR CLINICAL USE.