Recombinant Cascade Reagent (rCR) Endotoxin Detection Kit, 104 Tests (ES-0526-YC200)

Product Overview

The Recombinant Cascade Reagent (rCR) is an animal-free method used for the quantitative detection of gram-negative bacterial endotoxins (lipopolysaccharides, or LPS). rCR fully mimics the natural, multi-step enzymatic cascade found in the blue blood of the horseshoe crab (Limulus polyphemus), using three genetically engineered recombinant proteins that act sequentially to amplify the detection signal.
Step 1 Activation of Recombinant Factor C: The cascade begins when endotoxin molecules in a sample bind to inactive recombinant Factor C (rFC). This binding causes a conformational change that activates rFC into an active protease.
Step 2 Activation of Recombinant Factor B: The activated recombinant Factor C then targets inactive recombinant Factor B (rFB). It proteolytically cleaves rFB, converting it into its enzymatically active form.
Step 3 Activation of Recombinant Proclotting Enzyme: The activated recombinant Factor B in turn recognizes and cleaves the inactive recombinant proclotting enzyme, transforming it into the active recombinant clotting enzyme.
Our Recombinant Cascade Reagent Endotoxin Detection Kit (ES-0526-YC200) was designed to seamlessly replace traditional LAL. This kit offers an eco-sustainable, highly precise, and animal-free solution for your quantitative endotoxin testing needs.

Applications

Endotoxin testing

Sample Type

Biological products (e.g. antibody, protein)

Assay Type

Recombinant cascade reagent (rCR) assay (Quantitative)

Kit Contents

Recombinant cascade reagents (4 x 26 T/vial), Reconstitution buffer (12 mL/vial), Control standard endotoxin (2 vials), LAL reagent water (100 mL/vial)

Size

104 T

Storage

Store at 4°C.