AOF2 Polyclonal Antibody (NHP-AB303)

• This product is an antibody that was generated by immunizing rabbit against a portion of amino acids 100-150 of human LSD1. It is used only in in vitro assays.

Detailed Product Description

• Introduction: AOF2 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. AOF2 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. AOF2 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.
• Conjugate: None
• Applications: IHC; WB

Technical Specifications

• Host Species: Rabbit
• Species Reactivity: Dog; Human; Macaque; Monkey; Mouse; Rat; Zebrafish
• Immunogen: A portion of amino acids 100-150 of human LSD1

Product Property

• Purification: Immunoaffinity Chromatography
• Format: Liquid
• Concentration: 0.5 mg/mL
• Buffer: PBS, 0.2% gelatin, 0.05% sodium azide.
• Storage: Short term: 4˚C. Long term: Store at -20˚C.

Target Information

• Clonality: Polyclonal
• Alternative Names: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A; neuroprotective protein 3; RP1-184J9.1
• Entrez Gene ID: 23028