CD45RO Monoclonal Antibody (DO-9) (NHP-AB141)

• This product is an antibody that was generated by immunizing mouse against cultured human T-cells from an IL-2-dependent T-cell line (CA1). It is used only in in vitro assays.

Detailed Product Description

• Introduction: CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Conjugate: None
• Antibody Isotype: IgG2aκ
• Applications: Flow Cytometry; IF; IHC

Technical Specifications

• Host Species: Mouse
• Species Reactivity: Chimpanzee; Human
• Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1)

Product Property

• Purification: Protein G affinity chromatography
• Format: Liquid
• Concentration: 0.2 mg/mL
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Storage: Store at 4⁰C.

Target Information

• Target: CD45RO
• Clone: DO-9
• Clonality: Monoclonal
• Alternative Names: CD45; CD45 antigen; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
• Entrez Gene ID: 5788