Cas9 Expressing Monkey Primary Colonic Microvascular Endothelial Cells (NHFF-1123-HX36)

• Cas9-stable cells are seen as a popular research tool due to their capability of stably expressing Cas9 endonuclease, making a lot of contributions to gene editing experiments. We have developed a series of Cas9-stable cells including the Cas9-expressing monkey primary colonic microvascular endothelial cells by transducing Cas9-lentiviral particles. These cells are cultured in a gelatin pre-coated tissue culture flask until the third passage and then are collected in vials. You can expand them for 3-5 passages at a split ratio of 1:2 under a suitable culture condition. They are high-quality and highly customized and will help your project achieve more rapid progress.

Detailed Product Description

• Source: Healthy cynomolgus monkeys
• Purity: ≥ 80%
• Organ/Tissue: Colon
• Applications: Ideal for a wide range of CRISPR genome editing applications.

Technical Specifications

• Cell Type: Cas9 expressing cells
• Species: Cynomolgus monkey
• Cryopreservation: Cryopreserved with appropriate cryoprotectant.
• Quality Control: Rigorously control cell purity and viability.
• Cell Status: Cryopreserved

Shipping and Storage

• Shipping: Cryopreserved cells are shipped with dry ice overnight. Upon receipt, promptly transfer the frozen cells to liquid nitrogen for storage until they are ready for use. It is important to note that cells should never be stored in -20 °C or -80 °C freezer.
• Storage: Liquid nitrogen (-180°C)