Histone H2AX Polyclonal Antibody (NHP-AB373)

• This product is an antibody that was generated by immunizing rabbit against recombinant protein of human Histone H2AX. It is used only in in vitro assays.

Detailed Product Description

• Introduction: Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
• Conjugate: None
• Antibody Isotype: IgG
• Applications: ICC; IHC; WB

Technical Specifications

• Host Species: Rabbit
• Species Reactivity: Monkey
• Immunogen: Recombinant protein of human Histone H2AX

Product Property

• Purification: Affinity purification
• Format: Liquid
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Storage: Store at -20⁰C. Avoid freez-thaw cycles.

Target Information

• Clonality: Polyclonal
• Alternative Names: Gamma H2AX; H2A histone family, member X; H2a/x; H2AX histone; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX
• Entrez Gene ID: 3014