What is the specificity and sensitivity of the IFN-gamma Monoclonal Antibody (V23K9), PE?

The specificity and sensitivity of the IFN-gamma Monoclonal Antibody (V23K9), PE are critical for ensuring accurate detection of IFN-gamma in various biological samples. This antibody is highly specific to IFN-gamma, minimizing cross-reactivity with other cytokines. The conjugation with PE (phycoerythrin) enhances its sensitivity, providing robust fluorescence for flow cytometry analysis, making it suitable for various applications such as immune monitoring, functional assays, and cellular analysis.

What are the recommended storage conditions and shelf-life for the IFN-gamma Monoclonal Antibody (V23K9), PE?

Proper storage conditions are essential to maintain the efficacy and stability of the IFN-gamma Monoclonal Antibody (V23K9), PE. It is recommended to store the antibody at 2-8°C in the dark to preserve the PE conjugation. Avoid repeated freeze-thaw cycles as this can damage the antibody and reduce its binding efficiency. When stored under these conditions, the product typically has a shelf-life of up to 12 months, ensuring reliable performance throughout its usage period.

What are the optimal applications and performance characteristics of IFN-gamma Monoclonal Antibody (V23K9), PE?

The IFN-gamma Monoclonal Antibody (V23K9), PE, is primarily used in flow cytometry applications. This antibody is designed to detect IFN-gamma, a critical cytokine involved in immune responses, particularly produced by T-lymphocytes and natural killer (NK) cells. Key features and applications include: Flow Cytometry: The antibody is conjugated with PE (phycoerythrin), making it suitable for detecting IFN-gamma in flow cytometry assays. The PE conjugation provides bright and stable fluorescence, which is critical for accurate cell population analysis. Specificity: This antibody is highly specific to IFN-gamma, ensuring minimal cross-reactivity with other cytokines. This specificity is crucial for obtaining reliable and reproducible results in immunological studies.

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IFN-gamma Monoclonal Antibody (V23K9), PE (NHP-AB159)

This product is an antibody conjugated with PE that was generated by immunizing mouse against E. coli-expressed human IFN-gamma. It is used only in in vitro assays.

Detailed Product Description

Introduction: IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway.
Conjugate: None
Antibody Isotype: IgG1
Applications: Flow Cytometry

Technical Specifications

Host Species: Mouse
Species Reactivity: African green Monkey; Chimpanzee; Human
Immunogen: E. coli-expressed human IFN-gamma

Product Property

Format: Liquid
Buffer: Supplied in PBS
Storage: Store at 4⁰C.

Target Information

Target: IFN-gamma
Clone: V23K9
Clonality: Monoclonal
Related Disease: IFN gamma gene mutations are associated with aplastic anemia.
Alternative Names: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g
Entrez Gene ID: 3458

Downloadable Resources FAQs Customer Reviews

IFN-gamma Monoclonal Antibody (V23K9), PE (NHP-AB159) Data Sheet: Detailed information.

Q 1: What is the specificity and sensitivity of the IFN-gamma Monoclonal Antibody (V23K9), PE?
A: The specificity and sensitivity of the IFN-gamma Monoclonal Antibody (V23K9), PE are critical for ensuring accurate detection of IFN-gamma in various biological samples. This antibody is highly specific to IFN-gamma, minimizing cross-reactivity with other cytokines. The conjugation with PE (phycoerythrin) enhances its sensitivity, providing robust fluorescence for flow cytometry analysis, making it suitable for various applications such as immune monitoring, functional assays, and cellular analysis.
Q 2: What are the recommended storage conditions and shelf-life for the IFN-gamma Monoclonal Antibody (V23K9), PE?
A: Proper storage conditions are essential to maintain the efficacy and stability of the IFN-gamma Monoclonal Antibody (V23K9), PE. It is recommended to store the antibody at 2-8°C in the dark to preserve the PE conjugation. Avoid repeated freeze-thaw cycles as this can damage the antibody and reduce its binding efficiency. When stored under these conditions, the product typically has a shelf-life of up to 12 months, ensuring reliable performance throughout its usage period.
Q 3: What are the optimal applications and performance characteristics of IFN-gamma Monoclonal Antibody (V23K9), PE?
A: The IFN-gamma Monoclonal Antibody (V23K9), PE, is primarily used in flow cytometry applications. This antibody is designed to detect IFN-gamma, a critical cytokine involved in immune responses, particularly produced by T-lymphocytes and natural killer (NK) cells. Key features and applications include:
Flow Cytometry: The antibody is conjugated with PE (phycoerythrin), making it suitable for detecting IFN-gamma in flow cytometry assays. The PE conjugation provides bright and stable fluorescence, which is critical for accurate cell population analysis.
Specificity: This antibody is highly specific to IFN-gamma, ensuring minimal cross-reactivity with other cytokines. This specificity is crucial for obtaining reliable and reproducible results in immunological studies.

Exceptional Sensitivity & Specificity
I have been using the IFN-gamma Monoclonal Antibody (V23K9), PE, for flow cytometry experiments over the past few months. The sensitivity and specificity of this antibody have been exceptional, allowing me to detect IFN-gamma in my samples with great accuracy. The PE conjugation remains stable, providing consistent fluorescence without any notable signal degradation over time.
Reproducible Results
As an immunologist, I require antibodies that will deliver reproducible results, and the IFN-gamma Monoclonal Antibody (V23K9), PE, has not disappointed. The fluorescence intensity has been very stable, and I've had no issues with cross-reactivity. It's an excellent reagent for anyone needing reliable detection of IFN-gamma in their assays.

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