PCNA Polyclonal Antibody (NHP-AB414)

• This product is an antibody that was generated by immunizing rabbit against an internal region of PCNA (Human). It is used only in in vitro assays.

Detailed Product Description

• Introduction: PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
• Conjugate: None
• Applications: ELISA; IHC; WB

Technical Specifications

• Host Species: Rabbit
• Species Reactivity: Bovine; Chicken; Dog; Fish; Human; Monkey; Mouse; Rat; Xenopus
• Immunogen: An internal region of PCNA (Human)

Product Property

• Format: Liquid
• Concentration: 1 mg/mL
• Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
• Storage: Store at 4 or -20⁰C. Avoid freez-thaw cycles.

Target Information

• Clonality: Polyclonal
• Alternative Names: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen
• Entrez Gene ID: 5111