With our state-of-the-art analytical mass spectroscopy facility, Creative biolabs provides our global customers with customized in-depth conjugate site analysis of antibody-drug conjugates (ADCs). We guarantee top-quality analysis featuring comprehensive sequence coverage and accurate peptide identification.
Antibody-drug conjugates employ the specific monoclonal antibodies (mAbs) to achieve targeted delivery of the conjugated cytotoxic molecules to tumor cells. This delicate design increases the payload efficacy against tumor cells and in the meantime, reduces the cytotoxicity of the otherwise highly toxic payload to normal tissues comparing to traditional chemotherapeutic treatments. However, the ADCs are more complex and heterogeneous compared to the unmodified therapeutic mAbs. This is mainly due to a wide distribution of drug conjugation sites, the unique characteristics of the conjugation sites, and different types of conjugation chemistries. As a result, the effects of ADCs are remarkably different.
As one important aspect in ADC characterization, the identification of the conjugate sites provides an accurate map of the payload positions on a mAb. This set of results is extremely helpful in the prediction/validation of the effects on antigen binding activities induced by the conjugation process and in identification of bio-similarity ADC products. Conjugate sites analysis and determination is achieved by peptide mapping using highly sensitive and accurate LC-MS/MS.
After years of accumulative work, Creative biolabs has established a cutting-edge analytical mass spectroscopy platform to perform comprehensive analysis of the ADC conjugate sites. Generally, our analysis follows this peptide mapping workflow:
A schematic demonstration of ADC conjugate site analysis (left) and a sample report of the de-convoluted fragmentation spectra of peptides containing DM1 conjugated lysine (mAbs., 2016).
Creative biolabs is a pioneer in the development of antibody-drug conjugates. Based on years of efforts in therapeutic antibody discovery and synthetic organic chemistry, our Ph.D. level scientists are committed to provide our customers with one-stop services of their ADC development projects. Please contact us for more information and a detailed quote.
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