How is the beta-2 Microglobulin Polyclonal Antibody (KO Validated) utilized in research, and what are its key features?

The beta-2 Microglobulin Polyclonal Antibody is primarily used in immunohistochemistry (IHC), Western blotting (WB), and immunoprecipitation (IP) applications. It reacts with multiple species, including humans, mice, and rats, making it versatile for a wide range of research contexts. A significant aspect of this antibody is its recombinant production, which ensures batch-to-batch reproducibility, ethical standards compliance due to animal-free production, and confirmed specificity through extensive validation. These features contribute to its reliability in detecting beta-2 microglobulin across various experimental conditions, aiding in the exploration of its role in health and disease, particularly in studying cardiovascular diseases, cancer, and as a tumor biomarker.

Category: Non-Human Primate (NHP) related Antibody Products

beta 2 Microglobulin Polyclonal Antibody (KO Validated) (NHP-AB311)

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This product is an antibody that was generated by immunizing rabbit against recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1). It is used only in in vitro assays.

Introduction: Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

Conjugate: None

Antibody Isotype: IgG

Applications: IHC; IP; WB

Host Species: Rabbit

Species Reactivity: Human; Monkey; Mouse; Rat

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).

Purification: Affinity purification

Format: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage: Store at -20⁰C. Avoid freeze-thaw cycles.

Clonality: Polyclonal

Alternative Names: B-F; B2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3; Beta2 microglobulin; MHC Class I; microglobulin beta-2; Orla-B2m

Entrez Gene ID: 567

beta 2 Microglobulin Polyclonal Antibody (KO Validated) (NHP-AB311) Data Sheet: Detailed information. datasheet

Q 1: How is the beta-2 Microglobulin Polyclonal Antibody (KO Validated) utilized in research, and what are its key features?
A: The beta-2 Microglobulin Polyclonal Antibody is primarily used in immunohistochemistry (IHC), Western blotting (WB), and immunoprecipitation (IP) applications. It reacts with multiple species, including humans, mice, and rats, making it versatile for a wide range of research contexts. A significant aspect of this antibody is its recombinant production, which ensures batch-to-batch reproducibility, ethical standards compliance due to animal-free production, and confirmed specificity through extensive validation. These features contribute to its reliability in detecting beta-2 microglobulin across various experimental conditions, aiding in the exploration of its role in health and disease, particularly in studying cardiovascular diseases, cancer, and as a tumor biomarker.

Innovative and Reliable for Research Applications
This antibody's specificity and reliability across a range of experimental conditions, particularly in Western Blot and Immunohistochemistry, have made it an indispensable tool in our laboratory. The detailed purification notes and comprehensive application guidelines provided have significantly streamlined our protocols, enhancing the reproducibility of our results. Particularly impressive is its performance in detecting beta 2 Microglobulin in human urine samples, a testament to its high specificity and purity.
Exceptional Customer Support and Product Performance
After experiencing unexpected results in our initial experiments, the responsive and knowledgeable customer support team promptly addressed our concerns, providing valuable insights and alternative approaches to optimize our assays. The antibody's performance, notably its clean and specific band detection in Western Blot applications, has been pivotal in advancing our research on beta 2 Microglobulin's role in disease pathways. The commitment to quality and customer satisfaction is truly commendable.

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Fig.1 Obesity can be counteracted by gene regulation.

Your research is unique, and we understand that standard products may not always fit. Our team of experts specializes in custom NHP sample collections. We can design a tailored protocol to meet your specific research requirements, including samples with unique anticoagulant types, customized volumes, or from specific disease models.