Calnexin Polyclonal Antibody (NHP-AB317)

• This product is an antibody that was generated by immunizing rabbit against synthetic peptide derived from sequence near the amino-terminus of canine Calnexin, conjugated to KLH. It is used only in in vitro assays.

Detailed Product Description

• Introduction: Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
• Conjugate: None
• Applications: IHC; WB

Technical Specifications

• Host Species: Rabbit
• Species Reactivity: Guinea Pig; Hamster; Human; Monkey; Mouse; Pig; Rabbit; Rat; Sheep
• Immunogen: Synthetic peptide derived from sequence near the amino-terminus of canine Calnexin, conjugated to KLH.

Product Property

• Purification: Protein A Column
• Format: Liquid
• Concentration: 1 mg/mL
• Buffer: PBS, pH 7.2, 0.09% azide, 50% glycerol
• Storage: Short term: 4˚C. Long term: Store at -20˚C.

Target Information

• Clonality: Polyclonal
• Alternative Names: calmegin; Calnexin; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; p90; pp90
• Entrez Gene ID: 821