TAU (phospho Ser422) Polyclonal Antibody (NHP-AB273)

• This product is an antibody that was generated by immunizing rabbit against synthesized non-phosphopeptide derived from human Tau around the phosphorylation site of serine 422 (V-D-SP-P-Q). It is used only in in vitro assays.

Detailed Product Description

• Introduction: au is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
• Conjugate: None
• Antibody Isotype: IgG
• Applications: IHC; WB

Technical Specifications

• Host Species: Rabbit
• Species Reactivity: Bovine; Chicken; Chimpanzee; Dog; Gibbon; Goat; Gorilla; Human; Monkey; Mouse; Orangutan; Rabbit; Rat
• Immunogen: Synthesized non-phosphopeptide derived from human Tau around the phosphorylation site of serine 422 (V-D-SP-P-Q)

Product Property

• Purification: Immunoaffinity Chromatography
• Format: Liquid
• Concentration: 1 mg/mL
• Buffer: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
• Storage: Short term: 4˚C. Long term: Store at -20˚C. Avoid freeze-thaw cycles.

Target Information

• Clonality: Polyclonal
• Alternative Names: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau-4; Tau5
• Entrez Gene ID: 4137