Does the Caspase 3 Polyclonal Antibody react with multiple species, or is it specific to a certain species?

The Caspase 3 Polyclonal Antibody is designed to detect caspase-3 from a variety of species due to its broad reactivity profile. This antibody has been validated for use in human, mouse, and rat samples through western blot and immunohistochemistry assays. It is important for researchers to check the specific datasheet for additional species reactivity and validation details before making a purchasing decision.

What are the recommended protocols and conditions to achieve optimum performance and reliability using the Caspase 3 Polyclonal Antibody?

To ensure optimum performance of the Caspase 3 Polyclonal Antibody, it is recommended to follow the detailed protocol provided by Creative Biolabs. Proper antigen retrieval methods should be employed during IHC. It's also crucial to use appropriate positive controls and secondary antibodies conjugated with HRP for detection. Researchers should always run preliminary tests to optimize conditions specific to their experimental setup.

What are the optimal applications and dilutions for Caspase 3 Polyclonal Antibody?

The Caspase 3 Polyclonal Antibody is versatile and suitable for several applications including Western Blot (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC/IF). Western Blot (WB): The recommended dilution ranges from 1:500 to 1:2,500. This application is effective for detecting caspase-3 in various cell and tissue lysates. Immunohistochemistry (Paraffin) (IHC (P)): Optimal dilutions range from 1:500 to 1:1,000. The antibody can be used to stain paraffin-embedded tissue sections following antigen retrieval. Immunocytochemistry/Immunofluorescence (ICC/IF): Suggested dilutions range from 1:200 to 1:500 for detecting caspase-3 in fixed cells.

Category: Non-Human Primate (NHP) related Antibody Products

Caspase 3 Polyclonal Antibody (NHP-AB318)

Product Overview Detailed Product Description Technical Specifications Product Property Target Information Downloadable Resources FAQs Customer Reviews

This product is an antibody that was generated by immunizing rabbit against recombinant human Caspase-3 protein (full length). It is used only in in vitro assays.

Introduction: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Conjugate: None

Applications: IHC; WB

Host Species: Rabbit

Species Reactivity: Bovine; Chicken; Dog; Guinea Pig; Hamster; Human; Monkey; Mouse; Pig; Rabbit; Rat; Yeast

Immunogen: Recombinant human Caspase-3 protein (full length)

Purification: Protein A Column

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 0.09% azide, 50% glycerol

Storage: Short term: 4˚C. Long term: Store at -20˚C.

Clonality: Polyclonal

Alternative Names: Apopain; Apopain precursor; CASP-3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; Caspase3; CPP-32; Cysteine protease CPP32; ICE3; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1

Entrez Gene ID: 836

Caspase 3 Polyclonal Antibody (NHP-AB318) Data Sheet: Detailed information. datasheet

Q 1: Does the Caspase 3 Polyclonal Antibody react with multiple species, or is it specific to a certain species?
A: The Caspase 3 Polyclonal Antibody is designed to detect caspase-3 from a variety of species due to its broad reactivity profile. This antibody has been validated for use in human, mouse, and rat samples through western blot and immunohistochemistry assays. It is important for researchers to check the specific datasheet for additional species reactivity and validation details before making a purchasing decision.
Q 2: What are the recommended protocols and conditions to achieve optimum performance and reliability using the Caspase 3 Polyclonal Antibody?
A: To ensure optimum performance of the Caspase 3 Polyclonal Antibody, it is recommended to follow the detailed protocol provided by Creative Biolabs. Proper antigen retrieval methods should be employed during IHC. It's also crucial to use appropriate positive controls and secondary antibodies conjugated with HRP for detection. Researchers should always run preliminary tests to optimize conditions specific to their experimental setup.
Q 3: What are the optimal applications and dilutions for Caspase 3 Polyclonal Antibody?
A: The Caspase 3 Polyclonal Antibody is versatile and suitable for several applications including Western Blot (WB), Immunohistochemistry (IHC), and Immunocytochemistry (ICC/IF).
Western Blot (WB): The recommended dilution ranges from 1:500 to 1:2,500. This application is effective for detecting caspase-3 in various cell and tissue lysates.
Immunohistochemistry (Paraffin) (IHC (P)): Optimal dilutions range from 1:500 to 1:1,000. The antibody can be used to stain paraffin-embedded tissue sections following antigen retrieval.
Immunocytochemistry/Immunofluorescence (ICC/IF): Suggested dilutions range from 1:200 to 1:500 for detecting caspase-3 in fixed cells.

Outstanding Result
I've been using the Caspase 3 Polyclonal Antibody from Creative Biolabs in our apoptosis studies, and I must say the results have been outstanding. The antibody demonstrated high specificity and robustness across various assays, including Western blotting and immunohistochemistry. The consistency in performance has significantly accelerated our research. Also worth mentioning is the prompt and helpful customer service. I highly recommend this product for anyone working on cell death mechanisms.
Satisfied Performance
Our lab recently switched to the Caspase 3 Polyclonal Antibody from Creative Biolabs, and we've been very satisfied with its performance. The antibody is highly sensitive and provides clear, reproducible results in our Western blots and immunofluorescence experiments. It has proved to be a reliable reagent in detecting Caspase 3 in apoptotic pathways. The only minor drawback was a slight delay in shipping, but this was quickly resolved by their responsive customer support team. Overall, a great addition to our research tools.

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Fig.1 Obesity can be counteracted by gene regulation.

Your research is unique, and we understand that standard products may not always fit. Our team of experts specializes in custom NHP sample collections. We can design a tailored protocol to meet your specific research requirements, including samples with unique anticoagulant types, customized volumes, or from specific disease models.