Description
Basement Membrane Matrix, Phenol Red-Free is derived from mouse tumor basement membranes, containing essential ECM components like Laminin and Collagen IV, alongside vital growth factors. This standard formulation supports tumor invasion, angiogenesis, and organoid studies. The absence of phenol red eliminates color interference, making it ideal for colorimetric or fluorescent downstream assays requiring high optical clarity.
Organ/Tissue Related
General
Species Reactivity
Universal (Human, Mouse, etc. )
Application
Scaffold, Culture Support
Synonyms
Phenol Red-Free Basement Membrane Matrix; Basement Membrane Matrix without Phenol Red; Clear Basement Membrane Extract (Standard); ECM Gel, Phenol Red-Free Formulation; Standard Matrix for Organoids (No Phenol Red)
Gelling time
Room temperature: 5-30 min
Formulation
Formulated in DMEM supplemented with 50 µg/mL gentamicin.
Shipping
Shipping with dry ice
Storage
Store at -20°C for up to 2 years. Do not freeze-thaw repeatedly.
Precautions
1. Keep the product on ice in a 4°C refrigerator to thaw. Aliquot immediately after thawing and refreeze. 2. Use pre-cooled tips and tubes to avoid gelling. 3. Avoid warming the container with your hands. If the matrix semi-solidifies, return it to 0-4°C for 1-2 hours to restore fluidity. 4. Variations in color (straw yellow to dark red) are expected due to the pH indicator (phenol red) interacting with CO2 and do not impact performance. 5. For research use only. 6. Use appropriate personal protective equipment (lab coat and gloves).