NEFM Monoclonal Antibody (DO-9) (NHP-AB229)

• This product is an antibody that was generated by immunizing mouse against neurofilament preparation of calf brain tissue. It is used only in in vitro assays.

Detailed Product Description

• Introduction: Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function.
• Conjugate: None
• Antibody Isotype: IgG1
• Applications: Flow Cytometry; IHC; WB

Technical Specifications

• Host Species: Mouse
• Species Reactivity: Guinea Pig; Hamster; Human; Monkey; Rabbit; Xenopus
• Immunogen: Neurofilament preparation of calf brain tissue

Product Property

• Purification: Purified
• Format: Liquid
• Concentration: 1 mg/mL
• Buffer: PBS containing 0.09% sodium azide
• Storage: Short term: 4˚C. Long term: Store at -20˚C. Avoid freeze-thaw cycles.

Target Information

• Clone: DO-9
• Clonality: Monoclonal
• Alternative Names: 160 kDa neurofilament protein; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NF-M; NMC
• Entrez Gene ID: 4741