How specific is the PSMD4 Polyclonal Antibody, and what applications can it be used for in research?

The PSMD4 Polyclonal Antibody is typically designed to specifically detect the PSMD4 protein, which is a subunit of the 26S proteasome complex involved in the ubiquitin-proteasome pathway. This antibody could be validated for various applications such as Western blotting, immunoprecipitation, immunofluorescence, and possibly ELISA. Its specificity is usually determined by manufacturers through rigorous testing against both the target protein and non-specific proteins to ensure minimal cross-reactivity. Researchers are advised to look at the datasheet provided by the manufacturer for detailed validation information and recommended dilutions for different applications.

What are the recommended storage conditions for PSMD4 Polyclonal Antibody, and how stable is it over time?

The recommended storage conditions for the PSMD4 Polyclonal Antibody are typically at -20°C or -80°C for long-term storage to maintain its stability and activity. For short-term use, it can often be stored at 4°C. It is important to avoid repeated freeze-thaw cycles, as this can degrade the antibody and reduce its effectiveness. The stability of the antibody is provided by the manufacturer in terms of shelf-life, which is commonly about one year from the date of receipt when stored according to the instructions. Researchers should refer to the specific storage guidelines provided by the supplier to ensure optimal performance.

Category: Non-Human Primate (NHP) related Antibody Products

PSMD4 Polyclonal Antibody (NHP-AB434)

Product Overview Detailed Product Description Technical Specifications Product Property Target Information Downloadable Resources FAQs Customer Reviews

This product is an antibody that was generated by immunizing rabbit against recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1). It is used only in in vitro assays.

Introduction: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene.

Conjugate: None

Antibody Isotype: IgG

Applications: WB

Host Species: Rabbit

Species Reactivity: Human; Monkey; Mouse

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1)

Purification: Affinity purification

Format: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage: Store at -20⁰C. Avoid freeze-thaw cycles.

Clonality: Polyclonal

Alternative Names: 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; AF; angiocidin; anti secretory factor 1; antisecretory factor; Antisecretory factor 1; Multiubiquitin chain-binding protein; multiubiquitin-chain-binding protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; RP11-126K1.1; RPN10 homolog; S5a/antisecretory factor protein

Entrez Gene ID: 5710

PSMD4 Polyclonal Antibody (NHP-AB434) Data Sheet: Detailed information. datasheet

Q 1: How specific is the PSMD4 Polyclonal Antibody, and what applications can it be used for in research?
A: The PSMD4 Polyclonal Antibody is typically designed to specifically detect the PSMD4 protein, which is a subunit of the 26S proteasome complex involved in the ubiquitin-proteasome pathway. This antibody could be validated for various applications such as Western blotting, immunoprecipitation, immunofluorescence, and possibly ELISA. Its specificity is usually determined by manufacturers through rigorous testing against both the target protein and non-specific proteins to ensure minimal cross-reactivity. Researchers are advised to look at the datasheet provided by the manufacturer for detailed validation information and recommended dilutions for different applications.
Q 2: What are the recommended storage conditions for PSMD4 Polyclonal Antibody, and how stable is it over time?
A: The recommended storage conditions for the PSMD4 Polyclonal Antibody are typically at -20°C or -80°C for long-term storage to maintain its stability and activity. For short-term use, it can often be stored at 4°C. It is important to avoid repeated freeze-thaw cycles, as this can degrade the antibody and reduce its effectiveness. The stability of the antibody is provided by the manufacturer in terms of shelf-life, which is commonly about one year from the date of receipt when stored according to the instructions. Researchers should refer to the specific storage guidelines provided by the supplier to ensure optimal performance.

Satisfied Quality
As a cellular biology researcher, I have been extremely satisfied with the quality and performance of the PSMD4 Polyclonal Antibody from Creative Biolabs. The antibody has exhibited high specificity and sensitivity in our Western blot and immunoprecipitation assays. Consistent and reproducible results have made my project progress much smoother.
Robust Validation
I recently purchased the PSMD4 Polyclonal Antibody for our university's research on protein degradation mechanisms. From the packaging to the final application, the antibody surpassed our expectations. The robust validation provided by Creative Biolabs ensured we could trust its accuracy in both our immunofluorescence and ELISA tests. Additionally, the detailed datasheet and protocol recommendations were incredibly useful. Thanks to Creative Biolabs for such a high-quality reagent that significantly contributed to the success of our experiments!

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Fig.1 Obesity can be counteracted by gene regulation.

Your research is unique, and we understand that standard products may not always fit. Our team of experts specializes in custom NHP sample collections. We can design a tailored protocol to meet your specific research requirements, including samples with unique anticoagulant types, customized volumes, or from specific disease models.