- This product is an antibody that was generated by immunizing rabbit against recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1). It is used only in in vitro assays.
Detailed Product Description
- Introduction: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene.
- Conjugate: None
- Antibody Isotype: IgG
- Applications: WB
Technical Specifications
- Host Species: Rabbit
- Species Reactivity: Human; Monkey; Mouse
- Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1)
Product Property
- Purification: Affinity purification
- Format: Liquid
- Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
- Storage: Store at -20⁰C. Avoid freeze-thaw cycles.
Target Information
- Clonality: Polyclonal
- Alternative Names: 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; AF; angiocidin; anti secretory factor 1; antisecretory factor; Antisecretory factor 1; Multiubiquitin chain-binding protein; multiubiquitin-chain-binding protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; RP11-126K1.1; RPN10 homolog; S5a/antisecretory factor protein
- Entrez Gene ID: 5710
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Q 1: How specific is the PSMD4 Polyclonal Antibody, and what applications can it be used for in research?
A: The PSMD4 Polyclonal Antibody is typically designed to specifically detect the PSMD4 protein, which is a subunit of the 26S proteasome complex involved in the ubiquitin-proteasome pathway. This antibody could be validated for various applications such as Western blotting, immunoprecipitation, immunofluorescence, and possibly ELISA. Its specificity is usually determined by manufacturers through rigorous testing against both the target protein and non-specific proteins to ensure minimal cross-reactivity. Researchers are advised to look at the datasheet provided by the manufacturer for detailed validation information and recommended dilutions for different applications.
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Q 2: What are the recommended storage conditions for PSMD4 Polyclonal Antibody, and how stable is it over time?
A: The recommended storage conditions for the PSMD4 Polyclonal Antibody are typically at -20°C or -80°C for long-term storage to maintain its stability and activity. For short-term use, it can often be stored at 4°C. It is important to avoid repeated freeze-thaw cycles, as this can degrade the antibody and reduce its effectiveness. The stability of the antibody is provided by the manufacturer in terms of shelf-life, which is commonly about one year from the date of receipt when stored according to the instructions. Researchers should refer to the specific storage guidelines provided by the supplier to ensure optimal performance.
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Satisfied Quality
As a cellular biology researcher, I have been extremely satisfied with the quality and performance of the PSMD4 Polyclonal Antibody from Creative Biolabs. The antibody has exhibited high specificity and sensitivity in our Western blot and immunoprecipitation assays. Consistent and reproducible results have made my project progress much smoother. -
Robust Validation
I recently purchased the PSMD4 Polyclonal Antibody for our university's research on protein degradation mechanisms. From the packaging to the final application, the antibody surpassed our expectations. The robust validation provided by Creative Biolabs ensured we could trust its accuracy in both our immunofluorescence and ELISA tests. Additionally, the detailed datasheet and protocol recommendations were incredibly useful. Thanks to Creative Biolabs for such a high-quality reagent that significantly contributed to the success of our experiments!
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