Human Jo-1 Autoantibody (IgG) ELISA kit (CAT#: NAK-048)

This product is an ELISA kit for the determination of Jo-1 Autoantibody in human Plasma or Serum. It is highly sensitive and available for the detection of Jo-1 Autoantibody as low as 1.0 U/mL (12.5 to 200 U/mL).

Specifications
Sensitivity	1.0 U/mL (12.5 to 200 U/mL)
Assay Type	ELISA
Pack size	1×96 wells
Species Reactivity	Human
Antibody Type	IgG
Contents	Divisible microplate Calibrators (A-F) Control positive IgG (1) Control positive IgA (2) Sample buffer P (5x) Enzyme Conjugate TMB substrate Stop solution Wash solution (50x)
Sample Requirement	Plasma, Serum: 100ul
Storage	Short term: 4°C, Long term: See manual.

Target Information
Target	Jo-1 autoantibody
Introduction	Anti-Jo-1 antibody is a myositis specific autoantibody most commonly found in patients with idiopathic inflammatory myopathies (IIM). This antibody is directed against the histidyl-tRNA synthetase which catalyses the binding of the histidine to its cognate tRNA during protein synthesis. It can be considered a specific marker of IIM, predominantly found in 20-30% of patients with PM and in the 60-70% of those with interstitial pulmonary fibrosis. These antibodies are also found in DM, although less frequently than in PM, and are rare in children with PM or DM and in other connective tissue diseases.ELISA, CIE and immunoblotting are highly specific and sensitive techniques for testing anti-Jo-1 antibodies. The detection of this antibody is particularly useful in diagnosis and classification of IIM. Moreover, anti-Jo-1 serum levels strongly correlate with disease activity representing a good marker for disease monitoring.

Target Information

Target

Jo-1 autoantibody

Introduction

Anti-Jo-1 antibody is a myositis specific autoantibody most commonly found in patients with idiopathic inflammatory myopathies (IIM). This antibody is directed against the histidyl-tRNA synthetase which catalyses the binding of the histidine to its cognate tRNA during protein synthesis. It can be considered a specific marker of IIM, predominantly found in 20-30% of patients with PM and in the 60-70% of those with interstitial pulmonary fibrosis. These antibodies are also found in DM, although less frequently than in PM, and are rare in children with PM or DM and in other connective tissue diseases.ELISA, CIE and immunoblotting are highly specific and sensitive techniques for testing anti-Jo-1 antibodies. The detection of this antibody is particularly useful in diagnosis and classification of IIM. Moreover, anti-Jo-1 serum levels strongly correlate with disease activity representing a good marker for disease monitoring.

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For Research Use Only | Not For Clinical Use

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