NAA Profiling
NAA profiling technology is widely used to detect the autoantigens or autoantibodies (NAA) in patients with various diseases. It is a useful approach displaying significant advantages for the identification of biomarkers and for the prediction and diagnosis of diseases. Creative Biolabs is one of the well-recognized experts who are professional in NAA detection and analysis. With years of experience, our scientists can offer high-quality NAA profiling services to meet our clients’ demands precisely.
Introduction of NAA Profiling
NAA profiling technology is a high throughput approach to detect the autoantigens or autoantibodies existing in the body associated with various autoimmune diseases or dysregulation of the immune system. It is a powerful tool for the identification of biomarkers and for the prediction and diagnosis of diseases. In general, the NAA profiling is divided into the autoantigen microarray and autoantibody microarray.
1. Autoantigen Microarray
In autoantigen microarray, the autoepitopes (recombinant proteins or peptides) are spotted on microarrays and tested for Ig binding with test sera. Autoimmune disease-specific arrays inclusive of self-antigens, recombinant proteins, viral proteins, peptides and bacterial antigens have been validated for a number of autoimmune diseases including experimental autoimmune encephalomyelitis, multiple sclerosis, rheumatoid arthritis, diabetes, primary biliary cirrhosis, scleroderma and systemic lupus erythematosus. The potential applications of autoantigen microarrays are diverse and are not limited to the identification of “autoantibody signatures” associated with disease state or outcome, the profiling autoantibodies during the natural course of disease and monitoring change in the autoantibody profiles of patients in response to therapeutic intervention.
2. Autoantibody Microarray
Autoantibody is a type of antibody produced by the immune system that is directed against one or more of the individual's own proteins also called self-antigens. Autoantibody microarray detects the autoantibodies against a broad range of autoantigens is a powerful tool for the identification of biomarkers and for the prediction and diagnosis of diseases. Currently, the autoantibody microarray can be used to detect the IgG, IgM, IgA and IgE autoantibodies from serum or other body fluids. Firstly, the samples will be treated with DNAse I followed incubated with autoantigen array. Secondly, the autoantibodies binding to the antigens on the array, the images will be analyzed using special software. Lastly, the averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls.
Fig.1 A schematic overview of the autoantibody-autoantigen complex profiling array method. (Rho, 2013)
Why Choose Us?
- High rate of accuracy
- High throughput and high load
- High efficiency and reproducibility
- Timely and cost-effective
As one of the most professional service providers in antibody analysis, design and development, Creative Biolabs offers comprehensive NAA services to help you detect NAA repertoire using NAA profiling in a timely and cost-effective manner. Our customarily tailored services and high-quality products will contribute greatly to the success of your projects. Please feel free to contact us for more information and a detailed quote.
Reference:
- Rho, J.; Lampe, P.D. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays. Journal of Proteome Research. 2013, 12(5), 2311-20.
Choosing natural autoantibody (NAA) microarray to profile autoantibody repertoire and reveal novel disease's marker.