EBI3 Polyclonal Antibody (NHP-AB263)

• This product is an antibody that was generated by immunizing rabbit against a portion of amino acids 120-170 of human EBI3. It is used only in in vitro assays.

Detailed Product Description

• Introduction: EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively. IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis.
• Conjugate: None
• Applications: IHC; WB

Technical Specifications

• Host Species: Rabbit
• Species Reactivity: Chimpanzee; Human
• Immunogen: A portion of amino acids 120-170 of human EBI3

Product Property

• Purification: Protein G Column
• Format: Liquid
• Concentration: 0.5 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% sodium azide
• Storage: Short term: 4˚C. Long term: Store at -20˚C. Avoid freeze-thaw cycles.

Target Information

• Clonality: Polyclonal
• Alternative Names: cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; Epstein-Barr virus induced gene 3 (similar to Interleukin-27 beta chain precursor); Epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; IL-27 subunit beta; IL-27B; IL27 subunit; IL35 subunit; Interleukin-27 subunit beta
• Entrez Gene ID: 10148