How is PCNA Monoclonal Antibody utilized in DNA repair research?

It is essential for identifying and studying PCNA's role in DNA replication and repair processes, enabling researchers to understand cellular mechanisms fundamental to genetic stability.

Category: Non-Human Primate (NHP) related Antibody Products

PCNA Monoclonal Antibody (WA-19) (NHP-AB239)

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This product is an antibody that was generated by immunizing mouse against rat PCNA protein. It is used only in in vitro assays.

Introduction: PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

Conjugate: None

Antibody Isotype: IgG2aκ

Applications: Flow Cytometry; IF; IHC; WB

Host Species: Mouse

Species Reactivity: Chicken; Drosophila; Human; Monkey; Mouse; Pig; Rat; Yeast; Zebrafish

Immunogen: Rat PCNA protein

Purification: Protein G affinity chromatography

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Storage: Store at 4⁰C. Avoid freeze-thaw cycles.

Clone: WA-19

Clonality: Monoclonal

Alternative Names: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

Entrez Gene ID: 5111

PCNA Monoclonal Antibody (WA-19) (NHP-AB239) Data Sheet: Detailed information. datasheet

Q 1: How is PCNA Monoclonal Antibody utilized in DNA repair research?
A: It is essential for identifying and studying PCNA's role in DNA replication and repair processes, enabling researchers to understand cellular mechanisms fundamental to genetic stability.

Beyond The Expected Effectiveness
PCNA Monoclonal Antibody (WA-19) has been crucial for our lab's research on cell cycle regulation. The antibody's specificity and performance exceeded our expectations, providing clear and reproducible results in our experiments.
Outstanding Quality
The quality of the PCNA Monoclonal Antibody (WA-19) from Creative Biolabs is outstanding. It has significantly contributed to our studies on DNA repair mechanisms, offering consistent and reliable detection of PCNA in various assays.

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Fig.1 Obesity can be counteracted by gene regulation.

Your research is unique, and we understand that standard products may not always fit. Our team of experts specializes in custom NHP sample collections. We can design a tailored protocol to meet your specific research requirements, including samples with unique anticoagulant types, customized volumes, or from specific disease models.