Creative Biolabs develops and commercializes a full range of integrated innovative services that are based on phage display technology. We have ...
Creative Biolabs can offer advanced protein engineering platform for your specific project, including high-scale expression, crystallization and characterization...
Creative Biolabs has established custom membrane protein and membrane protein antibody production platforms for antibody discovery...
Creative Biolabs provides a full range of services based on our matured hybridoma platform. Our featured services involve the custom monoclonal antibody production, from ...
Creative Biolabs offers a unique yeast display technology (Ultraff™) for the discovery and optimization of monoclonal antibodies with pM affinity, the highest antibody affinity ever enabled by an in vitro recombinant antibody production technology. We have also created a next-generation, fully-human, yeast display scFv library for de novo discovery of therapeutically relevant antibodies.
Ultraff™ Yeast Display Technology has been invented as a novel technology for discovery and affinity maturation of fragment antibody, i.e. scFv and Fab, through the screening of an antibody library displayed on yeast surface. Yeast display (or yeast surface display or YDP) is a protein engineering technique widely used for in vitro protein evolution. Libraries of polypeptides displayed on the surface of yeast are screened using flow cytometry. Yeast is superior to phage for human antibody display in that the eukaryotic expression environment of yeast cells is more suitable for human protein folding, modification and translocation for display. Therefore, the yeast displayed antibodies are conformationally and functionally more close to human native counterparts.
The other advantage of Ultraff™ Yeast Display Technology is that antigen-binding yeast cell population can be easily sorted using Fluorescence Assisted Cell Sorting (FACS). This enables yeast display very efficient for affinity maturation because in additional to the antigen concentration management and stringent washing used in phage display, FACS is more efficient in direct sorting of higher affinity populations.
Fig. 1 Isolating and engineering human antibodies using yeast surface display. (Chao et al. 2006)
Creative Biolabs is also proud of a proprietary yeast transformation technique that guarantees up to 2×108 transformants per transformation. Previously, the low transformation efficiency of yeast cells significantly restrained the utilization of yeast display for antibody discovery. A typical efficiency of 105 transformants per transformation was nearly impossible to yield large size (over 109) libraries, which in turn, was hard to give rise to high affinity antibodies due to the limited diversity. With this yeast cell transformation technique, Ultraff™ Yeast Display Technology is now very efficient for high affinity antibody discovery and antibody affinity maturation.
Also, an UltraffTM human scFv antibody library has been created by combining a highly diverse collection of synthetically-constructed randomized CDR sequences that are further diversified by random lengths using a unique proprietary technique. The large library has been specifically optimized to eliminate unwanted stop codons and aggregation-prone sequences.
Customers own the exclusive rights to the antibodies derived from Ultraff™ Yeast Display Technology
Applications of Ultraff™ Yeast Display Technology:
Chao, G., Lau, W. L., Hackel, B. J., Sazinsky, S. L., Lippow, S. M. and Wittrup, K. D. (2006) 'Isolating and engineering human antibodies using yeast surface display', Nature protocols, 1(2), 755-768.
Our customer service representatives are available 24 hours a day, from Monday to Sunday. Contact Us