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The Turbidimetric Mechanism: When an endotoxin is introduced into the reconstituted LAL reagent, it activates a multi-stage proenzyme cascade that culminates in the activation of the core Clotting Enzyme. This enzyme cleaves the natural protein coagulogen present in the lysate, altering its structural solubility and causing the solution to become progressively turbid (cloudy) as it moves toward a physical gel state. An incubating microplate reader continuously tracks the light transmittance or absorbance of each well at 405 nm or 340 nm at regular intervals throughout the incubation process. The incubation software measures the time required for a specific well's optical density to rise to a pre-set absorbance threshold. Our Kinetic Turbidimetric Kit is engineered to provide exceptional sensitivity variations, allowing you to select the exact testing configuration that aligns with your specific sample matrix and regulatory threshold.
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