NAA Services for Anti-Cytokeratin 8
Creative Biolabs is objective to provide the widest range of products and services about NAA (natural autoantibodies) based on our years of experience and high-end technologies in NAA research. Our proven and optimized platforms can help you quickly get satisfactory results without repeated trials and win success in your NAA projects.
Background of Anti-Cytokeratin 8
Cytokeratin 8 or keratin-8 (CK8), fully named as keratin-type II cytoskeletal 8, is a keratin protein that helps to link the contractile apparatus to dystrophin at the costameres of striated muscle together with keratin 19. CK8, together with CK18, is the most common and characteristic component of the intermediate filaments in simple or single-layered epithelial tissues such as the liver and mammary gland, which also are the first cytokeratins expressed during embryogenesis. CK8 and CK18 play critical roles in biological activities, including regulation of the cell cycle and cytoprotective role against chemical insults. Abnormal expression of CK8 or CK18 often is considered as evidence of carcinogenesis, especially in the liver and breast.
Anti-CK8 are autoantibodies that react with an epitope on CK8, which have been demonstrated to be related to autoimmune disease and several disorders like pulmonary fibrosis, autoimmune hepatitis, and cancers.
Fig.1 The immunohistochemical localization of CK8 in cell cultures obtained from limbal epithelial cells. (Merjava, 2011)
The Role of Anti-Anti-Cytokeratin 8 in Primary Liver Cancer
As the most important form of primary liver cancer, hepatocellular carcinoma (HCC) is the fifth most important cancer in the world and the third most common cause of cancer death. In recent years, the incidence and mortality of HCC have increased. The high incidence of recurrence or metastasis after radical resection has led to the deterioration of the prognosis of HCC. Therefore, it is necessary and urgent to find poorly differentiated hepatocellular carcinomas and tumors in the early stage of HCC development.
It was implied in mouse models that CK8 and CK18 proteins were profoundly overexpressed in liver preneoplastic lesions, and more hepatocellular proliferative lesions can be detected in CK8/18 immunohistochemistry, which enabled CK8 and CK18 act as a reliable marker of and predict hepatocarcinogenesis and development. Anti- CK8 antibodies targeting on CK8, or together with CK18, may be useful immunohistochemical markers or diagnostic reagents in detection and evaluation of HCC.
Fig.2 Liver keratin phosphorylation in cirrhosis and normal liver. (Toivola, 2004)
With our rich experience in offering antibody and NAA services, scientists of Creative Biolabs are pleased to tailor the best-fit research program for your purpose. If you are working on NAA or interested in NAA research, please do not hesitate to contact us for more details.
References:
- Merjava, S.; et al. Cytokeratin 8 is expressed in human corneoconjunctival epithelium, particularly in limbal epithelial cells. Investigative Ophthalmology & Visual Science. 2011, 52(2): 787-794.
- Toivola, D.M.; et al. Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease. Hepatology. 2004, 40(2): 459-466.
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