What is Pironetin used for in biological research?

Pironetin is a microtubule inhibitor that disrupts the tubulin network, leading to cell cycle arrest and apoptosis. It is primarily used in cancer research to explore its potential as an anti-cancer agent.

How does Pironetin differ from other microtubule inhibitors?

Pironetin binds covalently to α-tubulin, causing destabilization of microtubules. Unlike other inhibitors like paclitaxel, which stabilize microtubules, Pironetin uniquely disrupts their function.

What concentration of Pironetin is typically used for cell assays?

Typical concentrations for cell-based assays range from 0.1 to 10 μM. However, the optimal concentration should be determined based on the specific cell line and experimental conditions.

What solvents are compatible with Pironetin for preparing solutions?

Pironetin can be dissolved in DMSO, ethanol, or other organic solvents. A final dilution in culture media or buffer is recommended to reduce solvent concentration in biological assays.

What solvents are compatible with Pironetin for preparing solutions?

Pironetin can be dissolved in DMSO, ethanol, or other organic solvents. A final dilution in culture media or buffer is recommended to reduce solvent concentration in biological assays.

Pironetin (CAT#: ADC-P-125)

ADCs Toxins, Pironetin is a potent inhibitor of tubulin. Pironetin covalently binds tubulin. Systematic alanine scanning shows, that the pironetin binding site was determined to be Lys352 of alpha-tubulin. Lys352 is located at the entrance of a small pocket of alpha-tubulin, and this pocket faces the beta-tubulin of the next dimer. This is the first compound that covalently binds to the alpha subunit of tubulin and Lys352 of alpha-tubulin and inhibits the interaction of tubulin heterodimers. HCT-116: IC50=0,002 uM; PSN1 : IC50=0,003 uM; T98G : IC50=15,43 uM; A549 : IC50=0,002 uM (preliminary laboratory results). Journal of Antibiotics (1996), 49, 173-180.

Product Information

CAS NO
151519-02-7

Description
ADCs Toxins, Pironetin is a potent inhibitor of tubulin. Pironetin covalently binds tubulin. Systematic alanine scanning shows, that the pironetin binding site was determined to be Lys352 of alpha-tubulin. Lys352 is located at the entrance of a small pocket of alpha-tubulin, and this pocket faces the beta-tubulin of the next dimer. This is the first compound that covalently binds to the alpha subunit of tubulin and Lys352 of alpha-tubulin and inhibits the interaction of tubulin heterodimers. HCT-116: IC50=0,002 uM; PSN1 : IC50=0,003 uM; T98G : IC50=15,43 uM; A549 : IC50=0,002 uM (preliminary laboratory results). Journal of Antibiotics (1996), 49, 173-180.

Classification
Microtubule Inhibitors

Molecular Weight
324.45

Purity
95%

Published Data

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Customer Reviews FAQ

Excellent

Creative Biolabs' Pironetin has been a powerful tool in our ADC research. Its potent inhibition of tubulin, specifically binding to Lys352, led to excellent cytotoxicity results across multiple cancer cell lines.

Excellent

Using Pironetin from Creative Biolabs delivered remarkable outcomes in our studies. The covalent binding to alpha-tubulin at Lys352 provided targeted and potent inhibition, significantly improving our results in ADC-based cancer research.

Excellent

Pironetin has proven to be an outstanding tubulin inhibitor in our lab. The high specificity and strong cytotoxicity across cancer lines made it a valuable component in our drug conjugate studies. Highly recommend Creative Biolabs' Pironetin.

Excellent

Pironetin by Creative Biolabs proved highly effective in our lab's cancer research. The covalent interaction with alpha-tubulin significantly disrupted tubulin dynamics, leading to strong anti-cancer effects in our cell line tests.

Excellent

We've seen great results with Pironetin from Creative Biolabs. Its unique tubulin-binding mechanism at Lys352 provided exceptional control in disrupting cancer cell proliferation, making it a valuable addition to our ongoing research.

Q: 1: What is Pironetin used for in biological research?
A: Pironetin is a microtubule inhibitor that disrupts the tubulin network, leading to cell cycle arrest and apoptosis. It is primarily used in cancer research to explore its potential as an anti-cancer agent.
Q: 2: How does Pironetin differ from other microtubule inhibitors?
A: Pironetin binds covalently to α-tubulin, causing destabilization of microtubules. Unlike other inhibitors like paclitaxel, which stabilize microtubules, Pironetin uniquely disrupts their function.
Q: 3: What concentration of Pironetin is typically used for cell assays?
A: Typical concentrations for cell-based assays range from 0.1 to 10 μM. However, the optimal concentration should be determined based on the specific cell line and experimental conditions.
Q: 4: What solvents are compatible with Pironetin for preparing solutions?
A: Pironetin can be dissolved in DMSO, ethanol, or other organic solvents. A final dilution in culture media or buffer is recommended to reduce solvent concentration in biological assays.
Q: 5: What solvents are compatible with Pironetin for preparing solutions?
A: Pironetin can be dissolved in DMSO, ethanol, or other organic solvents. A final dilution in culture media or buffer is recommended to reduce solvent concentration in biological assays.

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