Could the anti-Rat IgG(HL)Fab-N-MMAF ADC be used in combination with other therapeutic modalities?

Yes, this ADC can be combined with other therapeutic modalities, such as radiotherapy or other chemotherapeutic agents, to explore synergistic effects. Such combination therapies could potentially enhance overall treatment efficacy and overcome resistance mechanisms in target cells.

Can the ADC be used to study the mechanisms of antibody-dependent cellular cytotoxicity?

Yes, the anti-Rat IgG(HL)Fab-N-MMAF ADC can be utilized to study ADCC mechanisms by observing how effectively the ADC recruits and activates immune effector cells against target cells expressing rat IgG(H+L), offering valuable insights into immune-mediated therapeutic strategies.

What are the key benefits of using a secondary antibody like the Fab fragment in ADC design?

Employing a Fab fragment as a secondary antibody component enhances the versatility of the ADC. It allows for broad reactivity with various rat IgG(H+L) subtypes, facilitating its use in diverse experimental conditions and increasing its utility in preclinical screening processes.

How does the anti-Rat IgG(HL)Fab-N-MMAF ADC target specific cells within a biological system?

The ADC specifically targets cells that express or are coated with rat IgG(H+L), utilizing the Fab fragment's affinity for these antigens. This specificity ensures that MMAF is delivered directly to the intended cells, optimizing therapeutic efficacy and minimizing damage to healthy tissues.

In what types of cell culture models is the anti-Rat IgG(HL)Fab-N-MMAF ADC most effectively used?

The ADC is most effective in cell culture models that robustly express rat IgG(H+L), such as engineered tumor cell lines or primary cells from rat tissues. These models help in accurately assessing the ADC's binding efficiency, internalization rate, and cytotoxic effects.

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anti-Rat IgG(HL)Fab-N-MMAF ADC

anti-Rat IgG(HL)Fab-N-MMAF ADC (CAT#: ADC-AA-046)

This ADC product is comprised of a Fab fragment of an anti-rat IgG(H+L) specific polyclonal antibody conjugated via a noncleavable linker to MMAF. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, MMAF, is a cytotoxic small molecule which binds to tubulins, interrupts microtubule dynamics, and induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening rat monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG(H+L)

Overview
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system, which is a protein complex composed of four peptide chains—two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. IgG is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses.

Overview
Fab fragment of anti-rat IgG(H+L) specific polyclonal IgG antibody

Species Reactivity
Rat

Name
Noncleavable linkers

Description
Noncleavable linkers, is considered noncleavable-meaning linker cleavage, and payload release does not depend on the differential properties between the plasma and some cytoplasmic compartments. Instead, the release of the cytotoxic drug is postulated to occur after internalization of the ADC via antigen-mediated endocytosis and delivery to lysosomal compartment, where the antibody is degraded to the level of amino acids through intracellular proteolytic degradation.

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

The anti-Rat IgG(HL)Fab-N-MMAF ADC from Creative Biolabs is a game-changer. Its specificity and low toxicity make it ideal for pre-screening monoclonal antibodies. Highly satisfied with the results.

Excellent

Using this ADC product has streamlined our pre-screening process. The noncleavable linker and efficient targeting make it a reliable and cost-effective solution. Impressive performance!

Excellent

Creative Biolabs' anti-Rat IgG(HL)Fab-N-MMAF ADC exceeded our expectations. The product's low toxicity and high efficiency make it perfect for our experimental needs. Truly satisfied.

Excellent

This ADC product is a must-have for any lab working with rat monoclonal antibodies. The combination of a Fab fragment with MMAF offers outstanding results with no apparent toxicity.

Excellent

The anti-Rat IgG(HL)Fab-N-MMAF ADC has proven to be an excellent tool in our research. Its low toxicity profile and strong targeting capabilities make it a top choice. Great product!

Q: 1: Could the anti-Rat IgG(HL)Fab-N-MMAF ADC be used in combination with other therapeutic modalities?
A: Yes, this ADC can be combined with other therapeutic modalities, such as radiotherapy or other chemotherapeutic agents, to explore synergistic effects. Such combination therapies could potentially enhance overall treatment efficacy and overcome resistance mechanisms in target cells.
Q: 2: Can the ADC be used to study the mechanisms of antibody-dependent cellular cytotoxicity?
A: Yes, the anti-Rat IgG(HL)Fab-N-MMAF ADC can be utilized to study ADCC mechanisms by observing how effectively the ADC recruits and activates immune effector cells against target cells expressing rat IgG(H+L), offering valuable insights into immune-mediated therapeutic strategies.
Q: 3: What are the key benefits of using a secondary antibody like the Fab fragment in ADC design?
A: Employing a Fab fragment as a secondary antibody component enhances the versatility of the ADC. It allows for broad reactivity with various rat IgG(H+L) subtypes, facilitating its use in diverse experimental conditions and increasing its utility in preclinical screening processes.
Q: 4: How does the anti-Rat IgG(HL)Fab-N-MMAF ADC target specific cells within a biological system?
A: The ADC specifically targets cells that express or are coated with rat IgG(H+L), utilizing the Fab fragment's affinity for these antigens. This specificity ensures that MMAF is delivered directly to the intended cells, optimizing therapeutic efficacy and minimizing damage to healthy tissues.
Q: 5: In what types of cell culture models is the anti-Rat IgG(HL)Fab-N-MMAF ADC most effectively used?
A: The ADC is most effective in cell culture models that robustly express rat IgG(H+L), such as engineered tumor cell lines or primary cells from rat tissues. These models help in accurately assessing the ADC's binding efficiency, internalization rate, and cytotoxic effects.

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Other Products

Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-045	anti-Rat IgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-038	anti-RIgG(HL)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-034	anti-RIgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-035	anti-RIgG(HL)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-066	Anti-Rat IgG(H+L)-C-PBD ADC	Cleavable linkers	PBD (pyrrolobenzodiazepine)

CAT#	Product Name	Linker	Payload
ADC-AA-009	anti-HIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-008	anti-HIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-026	anti-MIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-063	Anti-HIgG(Fab)-N-MMAF ADC	Noncleavable linkers	MMAF
ADC-AA-019	anti-MIgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)

CAT#	Product Name	Linker	Payload
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-010	anti-HIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-491	Anti-TNFRSF17-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-053	Protein A-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-002	anti-HIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)

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