How does the noncleavable linker in the anti-RIgG(Fc)-N-MMAF ADC enhance its therapeutic efficacy?

The noncleavable linker in the anti-RIgG(Fc)-N-MMAF ADC provides increased stability in the bloodstream, ensuring that the cytotoxic agent MMAF remains attached to the antibody until it reaches and is internalized by the target cells. This minimizes premature release and reduces off-target toxicity, thereby enhancing therapeutic efficacy.

Can the anti-RIgG(Fc)-N-MMAF ADC be used in flow cytometry?

Yes, the anti-RIgG(Fc)-N-MMAF ADC can be utilized in flow cytometry experiments to evaluate the binding efficiency to cells expressing specific rabbit IgG Fc receptors. Proper controls should be included to validate the specificity and binding efficiency.

How does MMAF in the anti-RIgG(Fc)-N-MMAF ADC induce apoptosis in target cells?

MMAF, the cytotoxic component of the anti-RIgG(Fc)-N-MMAF ADC, binds to tubulin and disrupts microtubule dynamics, which is crucial for cell division. This disruption leads to cell cycle arrest and triggers apoptosis in the targeted cells.

Can the anti-RIgG(Fc)-N-MMAF ADC be used in preclinical models?

The anti-RIgG(Fc)-N-MMAF ADC is ideal for use in preclinical models to screen and evaluate rabbit monoclonal antibodies as potential ADC candidates. It provides a cost-effective and efficient approach for early-stage drug development.

What are the potential applications of the anti-RIgG(Fc)-N-MMAF ADC in cancer research?

The anti-RIgG(Fc)-N-MMAF ADC can be utilized in cancer research for targeting and eliminating cells expressing rabbit IgG Fc receptors. Its ability to deliver cytotoxic agents directly to cancer cells makes it a valuable tool for studying and developing new ADC-based therapies.

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anti-RIgG(Fc)-N-MMAF ADC

anti-RIgG(Fc)-N-MMAF ADC (CAT#: ADC-AA-032)

This ADC product is comprised of an anti-rabbit IgG Fc specific polyclonal antibody conjugated via a noncleavable linker to MMAF. The antibody portion is a secondary antibody and the drug portion, MMAF, is a cytotoxic small molecule which binds to tubulins, interrupts microtubule dynamics, and induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening rabbit monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
anti-rabbit IgG Fc specific polyclonal IgG antibody

Species Reactivity
Rabbit

Name
Noncleavable linkers

Description
Noncleavable linkers, is considered noncleavable-meaning linker cleavage, and payload release does not depend on the differential properties between the plasma and some cytoplasmic compartments. Instead, the release of the cytotoxic drug is postulated to occur after internalization of the ADC via antigen-mediated endocytosis and delivery to lysosomal compartment, where the antibody is degraded to the level of amino acids through intracellular proteolytic degradation.

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

The anti-RIgG(Fc)-N-MMAF ADC from Creative Biolabs provides exceptional specificity in targeting rabbit IgG. Its tubulin binding significantly enhances cytotoxic studies.

Excellent

Utilizing the anti-RIgG(Fc)-N-MMAF ADC has accelerated our pre-screening process for rabbit monoclonal antibodies. It's effective and shows no significant toxicity.

Excellent

Impressed by the robustness of anti-RIgG(Fc)-N-MMAF ADC in our lab. Its noncleavable MMAF linker delivers consistent results, crucial for our research.

Excellent

Creative Biolabs' anti-RIgG(Fc)-N-MMAF ADC has proven invaluable in our antibody development, offering a safe and efficient tool for rabbit antibody candidates.

Excellent

Using the anti-RIgG(Fc)-N-MMAF ADC, we've seen precise microtubule disruption in our targeted cells, making it a key component in our studies.

Q: 1: How does the noncleavable linker in the anti-RIgG(Fc)-N-MMAF ADC enhance its therapeutic efficacy?
A: The noncleavable linker in the anti-RIgG(Fc)-N-MMAF ADC provides increased stability in the bloodstream, ensuring that the cytotoxic agent MMAF remains attached to the antibody until it reaches and is internalized by the target cells. This minimizes premature release and reduces off-target toxicity, thereby enhancing therapeutic efficacy.
Q: 2: Can the anti-RIgG(Fc)-N-MMAF ADC be used in flow cytometry?
A: Yes, the anti-RIgG(Fc)-N-MMAF ADC can be utilized in flow cytometry experiments to evaluate the binding efficiency to cells expressing specific rabbit IgG Fc receptors. Proper controls should be included to validate the specificity and binding efficiency.
Q: 3: How does MMAF in the anti-RIgG(Fc)-N-MMAF ADC induce apoptosis in target cells?
A: MMAF, the cytotoxic component of the anti-RIgG(Fc)-N-MMAF ADC, binds to tubulin and disrupts microtubule dynamics, which is crucial for cell division. This disruption leads to cell cycle arrest and triggers apoptosis in the targeted cells.
Q: 4: Can the anti-RIgG(Fc)-N-MMAF ADC be used in preclinical models?
A: The anti-RIgG(Fc)-N-MMAF ADC is ideal for use in preclinical models to screen and evaluate rabbit monoclonal antibodies as potential ADC candidates. It provides a cost-effective and efficient approach for early-stage drug development.
Q: 5: What are the potential applications of the anti-RIgG(Fc)-N-MMAF ADC in cancer research?
A: The anti-RIgG(Fc)-N-MMAF ADC can be utilized in cancer research for targeting and eliminating cells expressing rabbit IgG Fc receptors. Its ability to deliver cytotoxic agents directly to cancer cells makes it a valuable tool for studying and developing new ADC-based therapies.

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Other Products

Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-055	Protein A-Duocarmycin ADC		Duocarmycins
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-048	Protein G-VC-MMAE ADC	VC (valine-citrulline)	MMAE (Monomethyl auristatin E)
ADC-AA-050	Protein G-MCC-DM1 ADC	MCC (Maleimidomethyl cyclohexane-1-carboxylate)	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)

CAT#	Product Name	Linker	Payload
ADC-AA-046	anti-Rat IgG(HL)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-001	anti-HIgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-018	anti-HIgG(Fab)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-008	anti-HIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-063	Anti-HIgG(Fab)-N-MMAF ADC	Noncleavable linkers	MMAF

CAT#	Product Name	Linker	Payload
ADC-W-493	Anti-CD19-Mc-MMAF ADC-3	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-010	anti-HIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-028	anti-MIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-W-491	Anti-TNFRSF17-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)

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