How does Anti-HIgG(Fc)Fab-C-PNU ADC specifically target cancer cells?

It targets cells expressing the Fc region of human IgG, making it highly specific for cells altered by disease processes involving IgG.

What is the cytotoxic mechanism of PNU-159682 in this ADC?

PNU-159682, a potent cytotoxin, binds to DNA within targeted cells, causing significant DNA damage that leads to cell death.

Why is a cleavable linker used in this ADC?

The cleavable linker ensures that PNU-159682 is released directly within the target cell, enhancing efficacy and reducing systemic toxicity.

What types of research is this ADC most suitable for?

It's ideal for screening potential human monoclonal antibodies in preclinical studies, determining their efficacy as ADC candidates.

How does the ADC's targeting ability benefit pre-screening processes?

It allows researchers to effectively assess monoclonal antibodies against a variety of IgG-expressing cells, speeding up candidate selection.

What advancements could further improve this ADC's research applications?

Enhancements could include optimizing linker stability, increasing PNU-159682's loading efficiency, and further refining the Fab fragment's specificity.

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anti-HIgG(Fc)Fab-C-PNU ADC

anti-HIgG(Fc)Fab-C-PNU ADC (CAT#: ADC-AA-014)

This ADC product is comprised of a Fab fragment of an anti-human IgG Fc specific polyclonal antibody conjugated via a cleavable linker to PNU-159682. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, PNU-159682, is a cytotoxic small molecule which binds to DNA, causes DNA damage. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening human monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Fab fragment of anti-human IgG Fc specific polyclonal antibody

Species Reactivity
Human

Name
Cleavable linkers

Description
Cleavable linkers rely on the physiological stimuli, which mainly include chemically cleavable linkers and enzymatically cleavable linkers. Chemically cleavable linkers including acid-labile linkers and disulfide linkers. For acid-labile linkers, intracellular release of payloads relies on the different pH between endosomes/lysosomes and blood. The release of disulﬁde-linked drugs is controlled by the factors in intracellular environment. Enzymatically cleavable linkers, peptide linkers and β-glucuronide linkers, are sensitive to enzymes located in cytoplasm.

Name
PNU-159682

Description
PNU-159682 is a major bioactive metabolite of Nemorubicin in human liver microsomes;> 3,000-fold cytotoxic than its parent compound (MMDX and doxorubicin).

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

The anti-HIgG(Fc)Fab-C-PNU ADC from Creative Biolabs has been pivotal in our ADC development. PNU-159682's potent DNA-binding capabilities make this an invaluable tool for effective and efficient pre-screening.

Excellent

Our team has seen remarkable results with the anti-HIgG(Fc)Fab-C-PNU ADC. The targeted DNA damage it induces is perfect for our stringent testing protocols, without causing undue toxicity.

Excellent

This product's precise delivery mechanism and minimal non-target toxicity have allowed us to advance our research with significant efficiency. The anti-HIgG(Fc)Fab-C-PNU ADC is a cornerstone in our lab for developing safer ADCs.

Excellent

Utilizing the anti-HIgG(Fc)Fab-C-PNU ADC has streamlined our screening process for monoclonal antibodies. It's not only effective but also economical, making it ideal for frequent use in our studies.

Excellent

The selective cytotoxicity of the anti-HIgG(Fc)Fab-C-PNU ADC is outstanding. It targets only the intended cells and minimizes collateral damage, which is crucial for our therapeutic studies.

Excellent

I've been impressed with the consistency and reliability of the anti-HIgG(Fc)Fab-C-PNU ADC. Its ability to cause DNA damage without primary antibody presence is particularly useful for our early-stage experiments.

Q: 1: How does Anti-HIgG(Fc)Fab-C-PNU ADC specifically target cancer cells?
A: It targets cells expressing the Fc region of human IgG, making it highly specific for cells altered by disease processes involving IgG.
Q: 2: What is the cytotoxic mechanism of PNU-159682 in this ADC?
A: PNU-159682, a potent cytotoxin, binds to DNA within targeted cells, causing significant DNA damage that leads to cell death.
Q: 3: Why is a cleavable linker used in this ADC?
A: The cleavable linker ensures that PNU-159682 is released directly within the target cell, enhancing efficacy and reducing systemic toxicity.
Q: 4: What types of research is this ADC most suitable for?
A: It's ideal for screening potential human monoclonal antibodies in preclinical studies, determining their efficacy as ADC candidates.
Q: 5: How does the ADC's targeting ability benefit pre-screening processes?
A: It allows researchers to effectively assess monoclonal antibodies against a variety of IgG-expressing cells, speeding up candidate selection.
Q: 6: What advancements could further improve this ADC's research applications?
A: Enhancements could include optimizing linker stability, increasing PNU-159682's loading efficiency, and further refining the Fab fragment's specificity.

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CAT#	Product Name	Linker	Payload
ADC-AA-025	anti-MIgG(Fc)-C-PNU ADC	Cleavable linkers	PNU-159682
ADC-AA-007	anti-HIgG(Fc)-C-PNU ADC	Cleavable linkers	PNU-159682

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