Exosome quantification is an important part before the downstream experiments. At present, the quantification of exosomes is mainly achieved by NTA (nanoparticle tracking analysis), but this method cannot distinguish the difference between exosomes and microvesicles or other particles. To solve such problems, fluorescent NTA (fNTA) was developed, which can accurately measure the concentration of exosomes in solution by staining exosomes and microvesicle plasmalemma; in addition, the concentration of exosomes can also be measured by flow cytometry. However, these methods are complicated to operate, costly, and require expensive equipment.
As a pioneer of exosome research, Creative Biolabs has developed a series of Exosome Capture & Quantification Kits that allow purification and quantification of circulating exosomes from various samples, such as human plasma, serum, urine or cell media. The kits use double antibody sandwich enzyme-linked immunosorbent assay (ELISA), specific total exosomes or exosome subpopulations can be simply and quickly captured and separated. The exosomes concentration in the samples can be measured by using the standard exosomes, and the expression changes of a protein in a large number of samples can be screened.