Plant-derived Exosome Research and Applications

Studying plant-derived exosomes can reveal the communication networks between plant cells, as well as the roles and regulatory mechanisms of these molecules in plant physiological processes, which is conducive to expanding their applications. Creative Biolabs is capable of providing a wide range of services related to plant-derived exosomes with its comprehensive exosome technology platform.

Functions of Plant-derived Exosomes

Plant-derived exosomes are involved in a variety of biological processes in their parental plants, such as growth and development, stress response, and protection against diseases.

Playing a role in how harmful microorganisms and plants interact

Exosomes isolated from the extracellular fluid of sunflower induced abnormalities and even death of fungal spores in terms of growth and development, morphology, etc., and it was proposed that their exosomes might play a role in how pathogenic microorganisms and plants interact.

Transporting lipids to resist biotic stress

The sphingolipids of Arabidopsis leaf-derived exosomes, which consist mainly of glycosyl inositol phosphate ceramides that can sense pathogenic fungi, are involved in the response of Arabidopsis to biotic stresses and in influencing its disease resistance.

Carrying small noncoding RNAs involved in plant immunity

Arabidopsis thaliana infested with Botrytis cinerea secretes exosomes containing small nRNAs that accumulate at the site of infection and deliver small nRNAs to the pathogen Botrytis cinerea, silencing pathogenicity-related genes.

Engaged in the synthesis of plant cell wall components

Picea asperata exosomes were observed in the secondary xylem and phloem and found to catalyze the hydrolysis of the corresponding polysaccharides by participating in the storage and transport of endo-1,4-β-glucanase, leading to modification of the cell wall.

Application as vectors

Delivery and expression of plant genes lead to the improvement of plant traits and quality.
The bioactive molecules in exosomes can also be used for drug delivery and disease research.

2-6-Plant-derived-Exosome-Research-and-Applications-1.png Fig. 1 The role of plant-derived exosomes in defense and symbiosis.¹

Research on Multiple Types of Plant-derived Exosomes

Types	Source examples	Research examples
Fruit-derived Exosome	Lemon, Strawberry, Grape, Coconut, etc.	Grape-derived exosomes have been found to alleviate dextran sodium sulfate-induced colitis by promoting the proliferation of intestinal stem cells, accelerating the regeneration of mucosal epithelial cells, and aiding in the restoration of the entire length and structure of the intestinal tract.
Flower-derived Exosome	Aloe vera, rose, tea, tea tree flower, etc.	Tea tree flower-derived exosomes inhibit mouse models of metastatic breast cancer by stimulating reactive oxygen species production causing mitochondrial damage and blocking the cancer cell cycle.
Medicinal Plant-derived Exosome	Ginseng, Asparagus cochinchinensis, mulberry bark, Phellinus linteus, etc.	Compared with other types of plant sources, medicinal plant-derived exosomes contain a large number of active small molecules originating from the donor itself, which can exhibit dual activity after loading drugs, with higher medicinal value. HJT-sRNA-m7-containing exosomes were extracted from Rhodiola rosea and confirmed to have potential anti-pulmonary fibrosis effects.
Vegetable-derived Exosome	Carrots, tomatoes, shiitake mushrooms, onions, etc.	Shiitake mushrooms-derived exosomes act as inhibitors of caspase 1 cleavage and IL-1β release, thereby blocking NLRP3 activation to reduce inflammation levels.
Cereal-derived Exosome	Wheat, oats, buckwheat, corn, etc.	Wheat-derived exosomes have significant pro-proliferative and migratory effects on endothelial, epithelial, and dermal fibroblasts, promoting the tube-forming ability of HUVEC cells

2-6-Plant-derived-Exosome-Research-and-Applications-2.png Fig. 2 Application of different plant-derived exosomes in the biotherapy of animal diseases.²

The research of plant-derived exosomes and their applications can provide a valuable resource for the development of innovative biotechnology and drug delivery systems. Creative Biolabs possesses years of experience as a supplier in the field of exosomes to meet the development and research goals of our clients for plant-derived exosomes. Please inquire us to learn more.

References

Cui, Yong, et al. "Plant extracellular vesicles." Protoplasma 257 (2020): 3-12.
Chen, Yu-Xin, and Qiang Cai. "Plant Exosome-like Nanovesicles and Their Role in the Innovative Delivery of RNA Therapeutics." Biomedicines 11.7 (2023): 1806.

Plant Exosome Isolation