For in vitro exosomal function exploration, exosome samples are usually labeled first, and the following researches will realize by tracking labeled exosomes. Creative Biolabs has established a comprehensive one-stop exosomal function in vitro research platform. Our services cover every step of the exploration, including the labeling and tracking, as well as the subsequent cell-based exosomal function researches.

Exosomes Labeling

• Fluorescent Dyes-Based Exosomes Labeling Service

In the published exosomes researches, most of the exosomes are labeled with lipophilic dyes, and they have many applications in vivo and in vitro. Lipophilic dyes are mainly divided into two categories. The first category is PKH67 (green fluorescence)/PKH26 (red fluorescence). Because they can stably bind to the lipid bilayer membrane of exosomes, and have better dyeing effects as well as the application. widely. The second category is the lipophilic dyes of the Di series, including DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence), and DiR (dark red fluorescence). Among them, the infrared fluorescence of DiR can penetrate cells and tissues and is used for tracking in in vivo imaging. In addition to lipophilic dyes that have been widely used, penetrant dyes, including carboxyfluorescein diacetate succinimidyl ester (CFSE) and calcein acetyl methyl ester (Calcein-AM), can penetrate exosomal membranes into the exosomes. CFSE and Calcein-AM can not only label exosomes but also verify the integrity of exosomes.

• Virus-mediated Labeling Exosomes Development Services

In this method, exosome-specific membrane proteins (such as CD9, CD63, and CD81) and fluorescent proteins are constructed as the expression elements into plasmids. Constructed plasmids are packaged into lentiviruses, and then the cells are infected with the lentiviruses so that the exosomes secreted by these cells are labeled by fluorescent proteins.

Fig.1 Different exosomal membrane proteins are involved in the fusion of targeting/labeling moieties for surface functionalization. (Salunkhe, 2020)

Add the labeled exosomes to the recipient cell culture medium and co-culture with the recipient cells to observe cell function changes, such as cell proliferation, migration, and invasion, cell apoptosis, etc.

Reference

1. Salunkhe, S.; Dheeraj.; et al. Surface functionalization of exosomes for target-specific delivery and in vivo imaging & tracking: Strategies and significance. Journal of Controlled Release. 2020. 326:599-614.

• Exosome Isolation
• Exosome Purification
• Exosome Characterization
• Exosome Quantification
• Exosome Profiling Services
• Exosomal Proteomic Detection Services
• Exosome Engineering Services
• Exosome Manufacturing Services
• Exosome Antibody Development Services
• Exosome Display
• Extracellular Vesicle Related Services
• Single-Cell & Exosome Combined Research
• Tissue Exosome Related Services
• Exosome Lipidomics & Metabolomics Services
• Exosome – NGS
• In Vivo Functional Services of Exosomes