Current studies on exosomes derived from cell supernatants or body fluids have limitations. Exosomes produced by cell line culture lack the complexity of the tissue microenvironment. In many liquid biopsy studies to screen for disease markers, researchers look for exosome biomarkers in the body fluids of patients, but various body fluids, especially blood, contain exosomes from multiple organs or tissue sources. These exosomes do not better represent the microenvironment of a specific tissue. Excitingly, some literature has reported the existence of exosomes in the interstitial space, and tissue exosomes will eventually reach the body fluid circulation. Therefore, exosomes directly isolated from tissue samples are more representative of changes in the microenvironment of diseased tissues in vivo than exosomes isolated from cell line culture or body fluids. In the future, tissue exosomes will have important clinical significance in the study of disease mechanisms and the screening of diagnostic markers, and their application prospects are huge. Referring to the current literature and multiple extraction experiences, Creative Biolabs has developed a method for extracting exosomes from tissues, which can provide efficient tissue exosome extraction and analysis services, and help customers mine the potential of exosomes from the most authentic environment.

Tissue Exosome Extraction Service at Creative Biolabs

The tissue exosomes we extract are authentic and exclusive, and the specific extraction process is mainly divided into four steps.

We can identify exosomes isolated from the interstitial space from the three aspects of particle size, morphology, and markers. The extracted tissue exosomes can be used for subsequent content analysis, exosome high-throughput sequencing , exosome proteomics, metabolomics and lipidomics.

Fig. 1 Biogenesis and identification of tissue-derived EVs (Ti-EVs). (Li, 2021)

Tissue Exosome Analysis Services at Creative Biolabs

After all, most of the exosomes in the interstitial space are secreted by different cells in the tissue, which is a heterogeneous collection of exosome subsets. So, in the process of extracting tissue exosomes, the dispersed multiple single cells carry this rich traceability information of exosomes. In recent years, with the rapid development of scientific research, single-cell sequencing has been widely used by researchers to study molecular information in a single cell. Therefore, we can help customers study the correlation between exosome subsets and cell subsets in tissues from the gene and protein levels, and advance exosome research to the level of individual exosomes. In addition, since the exosomes secreted into the interstitial space may flow into the body fluid circulation, we can provide combined research service on tissue exosomes and body fluid exosomes to help customers trace the exosomes from the tissue into the circulating body fluid. After locating the key molecules in exosomes through various high-throughput sequencing, we can verify the function of tissue exosomes for customers at the cell line, primary cell, or animal level. This can help customers explore the mechanism of action of exosomes from tissue, and provide a mechanistic basis for subsequent related therapeutic research.

• Body fluid exosome and tissue exosome combined research service
• Single-cell and tissue exosome combined transcriptomic research service
• Single-cell and tissue exosome combined proteomics research service
• Functional research and verification services for tissue exosome
• Therapeutic research service for tissue exosome

Creative Biolabs has rich experience in tissue processing and can provide a full range of research solutions for exosomes from various tissues. If you want to study the therapeutic or diagnostic potential of exosomes more realistically, please contact us with your sample specific information and specific requirements. Our professional team will provide customers with the most suitable experimental plan based on the previous exosome research experience, and promote the transformation of your ideas to the clinic.

Reference

1. Li, SR.; Man, QW.; et al. Tissue-derived extracellular vesicles in cancers and non-cancer diseases: present and future. Journal of Extracellular Vesicles. 2021. 10(14):e12175.

• Exosome Isolation
• Exosome Purification
• Exosome Characterization
• Exosome Quantification
• Exosome Profiling Services
• Exosomal Proteomic Detection Services
• Exosome Engineering Services
• Exosome Manufacturing Services
• Exosome Antibody Development Services
• Exosome Display
• Extracellular Vesicle Related Services
• Single-Cell & Exosome Combined Research
• Exosome Lipidomics & Metabolomics Services
• Exosome – NGS
• In Vitro Functional Services of Exosomes
• In Vivo Functional Services of Exosomes