Creative Biolabs has extensive experience to offer a variety of exosome services, including isolation, purification and characterization. In order to better understand the characteristics of exosomes and facilitate the later therapeutic and diagnostic application, we provide the detail size distribution and concentration measurement of exosomes based on the highly sensitive tunable resistive pulse sensing (TRPS) technology.

TRPS for Exosome Characterization

It is very essential to measure size distribution and concentration of exosomes when investigating their cellular function and uptake. Recently, the TRPS technology usually applied for a particle size distribution and concentration measurement has seen increased use for the characterization of exosome samples. TRPS is the most powerful nanoparticle characterization system available that allows high-throughput single particle measurements as colloids and/or biomolecular analytes driven through a size-tunable nanopore, one at a time.

The technique adapts the principle of resistive pulse-sensing, which monitors current flow through an aperture, combined with the use of tunable nanopore technology, allowing the passage of ionic current and particles to be regulated by adjusting the pore size. Particles crossing a pore are detected one at a time as a transient change in the ionic current flow, which is denoted as a blockade event with its amplitude denoted as the blockade magnitude. As blockade magnitude is proportional to particle size, accurate particle sizing can be achieved after calibration with a known standard. The main particle parameters of TRPS measurement is following:

  • Particle size and accurate number based size distribution, derived on a real particle by particle basis;
  • Particle concentration, being the number of particles/ml for a specified size range;
  • Particle charge and number based charge distribution, also derived on a real particle by particle basis.

TRPS (qNano) system.Fig.1 TRPS (qNano) system. (Anderson, 2015)

TRPS-based Exosome Characterization in Creative Biolabs

TRPS is an essential tool that is used to measure particle size and concentration, particularly in fields like exosome characterization. Unfortunately, TRPS measurements are susceptible to issues surrounding system stability, where the pore can become blocked by particles, and sensitivity issues, where particles are too small to be detected against the background noise of the system.

With the years of experience in exosome characterization and real-time update of technical date, Creative Biolabs improves the TRPS system sensitivity and stability by the optimization of system parameters and provides a comprehensive analysis of the parameters involved in exosome measurements such as size and concentration. We believe that our TRPS-based exosome characterization will help you better understand their physical characteristics, as well as their diagnostic application as potential sources of disease-related biomarkers.

Sectional schematic of a pore. Fig.2 Sectional schematic of a pore. (Blundell, 2015)

Our Superiority

  • TRPS system with sensitivity and stability
  • Comprehensive analysis of exosome characterization
  • Rich experience professionals' team
  • Whole-process technical trace service and direction

If you have any demands in exosome characterization, please don’t hesitate to contact us for more information.

References:

  1. Anderson, W.; et al. Observations of tunable resistive pulse sensing for exosome analysis: Improving system sensitivity and stability. Langmuir the Acs Journal of Surfaces & Colloids. 2015, 31(23):6577-87.
  2. Blundell, E.L.C.J.; et al. Emergence of tunable resistive pulse sensing as a biosensor. Analytical Methods. 2015, 7(17):7055-7066.

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