Overview

Wide Range of Viral Vectors for Exosome Labeling

Exosomes are extracellular vesicles released when multivesicular bodies within the cell fuse with the cellular membrane. Through genetic engineering of parental cells, the exosome membrane can be modified according to the mechanism of exosome generation. Fluorescent proteins are commonly used as reporter proteins, which emit a fluorescent signal when excited by light of a specific wavelength. By infecting parental cells with viral vectors carrying sequences for both fluorescent proteins and exosomal membrane proteins, these cells can be genetically modified to secrete exosomes that contain fluorescent proteins. This enables the tracking of exosomes as they move from donor to recipient cells.

Services

Creative Biolabs offers a variety of viral vectors that can be used to infect parental cells directly, assisting customers in tracking exosome movement.

Extensive Selection of Viral Vectors for Exosome Labeling

Replication-defective recombinant viruses, such as lentiviruses with deleted virulence-related genes, are widely used in genetic engineering. These viruses can efficiently introduce and integrate exogenous genes into the genome of target cells through in vitro infection, ensuring long-term expression of the genes in both dividing and non-dividing cells. Lentiviruses have the ability to infect a broad range of host cells, including tumor cell lines, fibroblasts, neurons, lymphocytes, and macrophages. The tetraspanin family proteins, such as CD63, CD81, and CD9, are highly expressed on exosomal membranes and are involved in exosome trafficking. By incorporating these exosomal membrane proteins along with fluorescent proteins into lentiviral vectors, the vectors can be used to infect parental cells. The fluorescent membrane proteins expressed on the exosome membrane can then be utilized to track the formation, secretion, targeting, and trafficking mechanisms of exosomes.

Fig.1 Images of HEK293T cells transfected with the CD63-EGFP plasmid at 24- and 48-hours post-transfection.

Fig.2 EGFP-labeled exosome identification by transmission electron microscopy (A), nanoparticle tracking analysis (B), and nano-flow cytometry (C).

Features

• The lentiviral vectors developed by Creative Biolabs can express fusion proteins through the CMV promoter in most mammalian cells.
• These lentiviral vectors have high biological safety, a wide range of hosts, and high lentiviral titers, which can reach 10^9 TU/mL.
• Creative Biolabs provides lentiviral vectors and purified fluorescent exosomes carrying different fluorescent markers (including (E)GFP, BFP, and mCherry), which can meet customers' requirements for color.
• If you have innovative ideas and needs, Creative Biolabs can provide you with personalized fluorescent exosome preparation services.

Fig.3 Fusion strategy of exosomal membrane proteins and fluorescent proteins.

Creative Biolabs is the leading provider of exosome services and products. We can provide one-stop services including exosome extraction, exosome identification, exosome modification, exosome profiling, and exosome function verification in vivo and in vitro. Please contact us to put forward your needs and ideas to provide you with the most suitable products, or formulate the most suitable solutions.

FAQs

• Fluorescent Dye based Exosome Labeling