Although some markers from body fluid-derived exosomes have been identified for disease diagnosis and prognosis, body fluid exosomes are still a mixed set containing exosomes from various tissues and cells in the body, and they cannot completely represent real changes in lesion sites. Creative Biolabs has formed a unique tissue exosome extraction process, based on which it can provide combined research service for tissue exosomes and body fluid exosomes, helping customers to screen out diagnostic markers that can be transformed into clinical practice and developed products that provide convenience for human health.

Starting Point for Body Fluid Exosome and Tissue Exosome Combined Research Service

At present, tissue biopsy is still used in the clinical diagnosis of some diseases, but this method is limited in the application and promotion of early screening of clinical tumors. As a brand-new method, liquid biopsy based on body fluid avoids the harm of surgery to patients and has great advantages and great application potential. However, the sensitivity and specificity of existing liquid biopsy markers are still far from meeting the needs for precise diagnosis and treatment. Due to the variety of information carried, the key information in body fluid exosomes has great potential as a diagnostic marker for diseases. However, body fluid exosomes originate from various tissues in the body, resulting in a complex composition. Therefore, the proportion of cell-derived exosomes in the real lesion site is very low. Overall, it remains unclear whether markers in body fluid exosomes screened by omics analysis are of disease-specific origin. It is an urgent problem to find markers with high accuracy and reliability in body fluid exosomes.

Fig. 1 Advantages and disadvantages of liquid versus tissue biopsy. (Nikanjam, 2022)

Tissue exosomes are a type of exosomes that are secreted by various cells in specific tissues into the interstitial space and carry tissue-specific information. These exosomes can enter the body fluid circulation to transmit signals to target organs or tissues. The most important thing is that tissue-derived exosomes contain more rich information on the lesion site and can better reflect the real situation of the lesion site. With the continuous improvement of tissue exosome extraction technology, some researchers have screened out some key molecules in exosomes that exist in body fluids and diseased tissue microenvironments. This helps to more precisely find exosome-based liquid biopsy markers.

Body Fluid Exosome and Tissue Exosome Combined Research Process

Several research teams took the lead in isolating extracellular vesicles and particles (EVPs) from different tumor tissues and several body fluid sources for proteomics. Then they performed a joint analysis of EVPs from these different sources to screen some potential biomarkers in exosomes for tumor detection and determination of tumor types. Since the vesicles were isolated in an ultracentrifuge manner in this study, some non-vesicle particles still exist, so the definition of EVPs was used to describe it more accurately. At Creative Biolabs, we can provide customers with the tissue exosome extracting service successively through differential centrifugation, ultracentrifugation, molecular size exclusion, and ultrafiltration. More importantly, our professional bioinformatics analysis team can provide customers with a more authoritative marker collection in exosomes through a variety of calculation methods and comparative analysis.

Fig. 2 Extracellular vesicle and particle biomarkers define multiple human cancers. (Hoshino, 2020)

Creative Biolabs has been focusing on exosome research and providing customers with the most advanced exosome technology services for many years. In addition to innovative tissue exosome extraction services and exosome extraction services suitable for various body fluids, we can also provide subsequent exosome identification, exosome proteomics, exosome next-generation sequencing, exosome lipidomics, metabolomics, etc. If you want to find more reliable disease markers in body fluid-derived exosomes, please contact us to inform us of your specific sample information and research purposes. We will reply to your inquiry the first time.

References

1. Nikanjam, M.; Kato, S.; et al. Liquid biopsy: current technology and clinical applications. Journal of Hematology & Oncology. 2022. 15(1):131.
2. Hoshino, A.; Kim, HS.; et al. Extracellular vesicle and particle biomarkers define multiple human cancers. Cell. 2020. 182(4):1044-1061.

• Single-Cell & Tissue Exosome Combined Transcriptomic Research
• Single-Cell & Tissue Exosome Combined Proteomics Research
• Tissue Exosome Related Functional Research and Verification
• Tissue Exosome Related Therapeutic Research