Single-Cell & Tissue Exosome Combined Proteomics Research Service

Single-cell transcriptomics in the single-cell detection field has been widely used in analyzing cell heterogeneity and cell subpopulation function. However, changes in RNA levels may not ultimately feedback on the true state of the cell. Since protein is the end product of genetic information transmission and the main bearer of life activities, single-cell proteomics can better reflect the physiological and pathological state of cell subgroups than single-cell transcriptomics. Almost all cells are capable of intercellular communication by secreting exosomes encapsulated with various bioactive molecules. In particular, tissue exosomes are a collection of exosomes secreted by different cells in the tissue in the interstitial space. This collection can more truly reflect the intercellular regulatory mechanism in the lesion. However, the heterogeneity of tissue exosomes prevents a clearer elucidation of which cells produce which key molecules in exosomes and what functions they have. Creative Biolabs can provide innovative single-cell and tissue exosome combined proteomics service, helping customers to elucidate the exosome regulatory network in different cells in a tissue or between different tissues from an important protein level.

Single-Cell Proteomics

Single-cell proteomics is the study of protein expression profiles of cell subpopulations through qualitative and quantitative analysis of proteins from the single-cell dimension. There are already a variety of single-cell proteome analysis methods in different subdivisions, including single-cell mass cytometry based on antibody labeling, single-cell functional protein analysis based on cytokine panel detection, and single-cell protein quantification based on TMT markers spectrum technology. These techniques enable the clustering of cell subpopulations from protein clustering at different scales. At Creative Biolabs, we can isolate cell collection and exosome collection by providing efficient tissue permeabilization techniques. On this basis, through the most suitable single-cell proteomics and exosome proteomics, we can find the parental cell types of key proteins in exosomes for customers.

Fig. 1 Global overview of the single-cell proteomics workflow analyzed by mass spectrometry. (Arias-Hidalgo, 2022)

Single-Cell and Tissue Exosome Combined Proteomics Research Prospects

Among the many cargoes carried by exosomes, proteins are an important class of signaling molecules, which can directly participate in the regulation of various pathways and affect the function of recipient cells. Some studies have found possible donor cells of tissue exosomes through single-cell transcriptomics and exosome transcriptomics and proposed a possible transport mechanism for tissue exosomes. Similarly, the emergence of single-cell proteomics can also more accurately determine the traceability and communication mechanism of tissue exosomes from the protein level. The combined proteomics research of single-cell and tissue exosomes will greatly promote the in-depth analysis of the exosomal protein mechanism behind the life phenomena, and help researchers achieve high-quality and high-level research results.

In line with the original intention of providing customers with high-quality and efficient exosome technical services, Creative Biolabs has been paying attention to the latest developments in the exosome research field and continues to carry out technology development, process optimization, equipment introduction, and recruitment of outstanding talents, aiming to provide customers with high-end one-stop technical service for exosome research. You only need to provide tissue samples, and our professional technical team can provide you with efficient tissue exosome extraction service, tissue exosome identification service, single-cell and tissue exosome combined proteomics service, and subsequent in vivo and in vitro functional mechanism verification service. Please contact us with your specific requirements, we look forward to working with you to explore the tissue-specific exosome communication network at the protein level.

Reference

Arias-Hidalgo, C.; Juanes-Velasco, P.; et al. Single-cell proteomics: the critical role of nanotechnology. International Journal of Molecular Sciences. 2022. 23(12).

Plant Exosome Isolation