Tissue Exosome Related Therapeutic Research Service

Research hotspots in the field of exosomes have been focused on cellular supernatant sources and humoral sources, with some limitations for both. Yet, tissue exosomes have received increasing attention from researchers for their unique potential to provide a realistic and specific reflection of the local tissue microenvironmental exosomal conditions. In addition to investigating the characterization of contents, the development of diagnostic markers, and their biological functions with tissue exosomes, their therapeutic properties are also an attractive research direction. Creative Biolabs is dedicated to digging deeper into the applied research thoughts of tissue exosomes and can provide services related to the therapeutic research of tissue exosomes.

Fig.1 Tissue-derived EVs in clinical practice. (Li, 2021)

The therapeutic applications of tissue exosomes have been the subject of cutting-edge research in several areas, including but not limited to:

Tissue Exosomes in Combination with Therapeutically Agents

Gram-negative bacteria naturally produce outer membrane vesicles capable of naturally carrying immunostimulatory proteins, but their vesicle proteins are at risk of triggering severe innate immune responses. The researchers optimized their production protocol and combined it with tumor tissue exosomes, resulting in the preparation of attenuated outer membrane vesicles that exert the potential to modulate the tumor immunosuppressive microenvironment. Also, in this synthetic bacterial vesicle, tumor exosome combination anti-PD-1 therapeutic system, bacterial vesicles induced tumor-specific adaptive immunity and initiated tumor exosome-driven Th1 immune responses, exhibiting a stronger synergistic response than that induced by each component acting alone.

Tissue Exosomes for Injury Repair

Researchers isolated liver tissue-derived exosomes that inhibited the progression of CCl4-induced acute hepatotoxicity and blocked the apoptotic pathway in liver tissue. The proliferation of hepatocytes and activation of hepatic stellate cells were observed in areas of hepatic necrosis in the mouse model after liver tissue exosome treatment, indicating accelerated recovery of hepatic necrotic areas. Thus, another direction for therapeutic research of tissue-derived exosomes is their vital role in maintaining homeostasis after tissue injury.

Fig.2 Liver tissue exosomes accelerate recovery of hepatic necrosis. (Lee, 2021)

Tumor Tissue Exosomes as Pro-regenerative Therapeutic Agents

Tissue exosomes also have therapeutic potential for tissue regeneration. For example, it was confirmed that lipoma tissue-derived exosomes and adipose tissue-derived exosomes have similar lipogenic functions, facilitating the search for new cell-free tissue regeneration therapies. This was demonstrated by in vitro experiments analyzing the phenotypic changes in exosome uptake, proliferation, migration, and lipogenic differentiation of adipose tissue stem cells, as well as exosomes recruiting adipocytes in rat adipose grafts to promote angiogenesis and adipogenesis.

Optimizing the isolation methods of tissue exosomes to obtain purer samples and establishing standardized protocols for their characterization and potency analysis are essential to confirm the therapeutic activity and mechanism. In addition, tissue exosomes have the potential to be modified to improve their targeting function and therapeutic efficacy. Creative Biolabs has established a stable and mature platform for engineering exosomes and can provide services for therapeutic studies of tissue exosomes in various animal models. Please feel free to contact us.

References

Li, S.R.; et al. Tissue-derived extracellular vesicles in cancers and non-cancer diseases: Present and future. J Extracell Vesicles. 2021, 10(14): e12175.
Lee, J.; et al. Extracellular vesicles from in vivo liver tissue accelerate recovery of liver necrosis induced by carbon tetrachloride. J Extracell Vesicles. 2021, 10(10): e12133.

Plant Exosome Isolation