Exosome Marker related Antibody Products

Products Overview Features FAQs

Overview

The classical exosome markers include CD9, CD63, CD81, Alix, and TSG101. These proteins are widely used in the isolation, purification, and identification of exosomes and have been recognized as standard markers in exosome research. In addition to these classical markers, other proteins such as CD326 (EpCAM), Caveolin-1, CD41, CD44, Flotillin 1, Rab5, TM9SF4, TM9SF3, HSC70, and HSP70 are also widely utilized in various studies, particularly in the analysis of exosomes from specific cell types or functional exosomes. These proteins are closely related to the formation, transport, and interaction of exosomes with target cells, making them highly significant in exosome-related research.

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Creative Biolabs offers high-quality antibodies covering these marker proteins, supporting customers in the comprehensive characterization and study of exosomes in a variety of experiments. Whether through Western Blotting, ELISA, nano-flow cytometry, or immunofluorescence techniques, customers can use these antibodies for qualitative and quantitative analysis, as well as functional studies of exosomes.

Features

Comprehensive coverage of exosome marker proteins
Broad applicability across various experimental techniques
High specificity and sensitivity
Customized antibody labeling services available

FAQs

Q: Are there suitable antibodies available for exosome nano-flow cytometry analysis?

A: Yes, our product list includes FITC-labeled antibodies against exosome surface proteins, which are suitable for flow cytometry analysis of exosomes. Please ensure that the antibody's species reactivity matches your sample.

Q: How can I ensure that only exosomes, and not other cellular debris, are detected in the experiment?

A: To ensure specificity, first focus on the purity of the exosome sample using extraction methods, NTA, and TEM. Additionally, using antibodies against exosome-specific markers (e.g., CD9, CD63, CD81, TSG101) along with the exosome-negative marker Calnexin can help further confirm the presence and purity of exosomes.

Q: How can I avoid background interference or blurred bands in Western blotting?

A: Background interference may result from high antibody concentration, incomplete blocking, or insufficient washing. Optimizing the antibody concentration and incubation time, along with thorough blocking and adequate washing steps, can effectively reduce background. Blurred bands may be caused by sample degradation or suboptimal electrophoresis conditions; using fresh samples and optimizing SDS-PAGE conditions are recommended.

Q: Why is the target protein not detected when using exosome marker antibodies?

A: Failure to detect the target protein could be due to low expression levels of the target protein in the sample or issues with the experimental steps. Increasing the concentration of the exosome sample may help raise the target protein level. Additionally, optimizing the antibody incubation time and concentration may improve detection efficiency.

Products

Cat	Product Name	Host Animal	Cross Reactivity	Application	Conjugate	Size
EXA-0822-X220	Mouse Anti-Human CD9 Monoclonal Antibody	Mouse	Human	ELISA, WB, IHC
EXA-0822-X221	Rabbit Anti-Human CD9 Monoclonal Antibody	Rabbit	Human	ELISA, WB, IHC, IF, FC
EXA-0822-X222	Rabbit Anti-Human CD63 Polyclonal Antibody	Rabbit	Human	WB, ELISA
EXA-0822-X223	Anti-Human CD81 Monoclonal Antibody		Human	ELISA
EXA-0822-X224	Rabbit Anti-Human, Mouse, Rat CD81 Monoclonal Antibody	Rabbit	Human, Mouse, Rat	ELISA, WB, IHC, FC
EXA-0822-X225	Rabbit Anti-Human, Rat CD326 Polyclonal Antibody	Rabbit	Human, Rat	ELISA, WB, IHC, IF
EXA-0822-X226	Rabbit Anti-Human, Mouse, Rat CD9 Polyclonal Antibody	Rabbit	Human, Mouse, Rat	ELISA, WB, IHC, IF		100 µL
EXA-0822-X227	Rabbit Anti-Human CD9 Polyclonal Antibody [HRP]	Rabbit	Human	ELISA	HRP
EXA-0822-X228	Mouse Anti-Human, Rabbit CD63 Monoclonal Antibody	Mouse	Human, Rabbit	ELISA, WB, IHC, IF, FC
EXA-0822-X229	Rabbit Anti-Human,Mouse CD63 Polyclonal Antibody	Rabbit	Human,Mouse	ELISA,WB,IHC
EXA-0822-X230	Mouse Anti-Human CD63 Monoclonal Antibody	Mouse	Human	ELISA, WB, IHC, IF, FC
EXA-0822-X231	Rabbit Anti-Mouse CD63 Polyclonal Antibody [HRP]	Rabbit	Mouse	ELISA	HRP
EXA-0822-X232	Mouse Anti-Human CD81 Monoclonal Antibody	Mouse	Human	ELISA,IHC
EXA-0822-X233	Rabbit Anti-Mouse CD81 Polyclonal Antibody [HRP]	Rabbit	Mouse	ELISA	HRP
EXA-0822-X234	Rabbit Anti-Mouse CD81 Polyclonal Antibody [Biotin]	Rabbit	Mouse	ELISA	Biotin
EXA-0822-X239	Rabbit Anti-Human CD9 Polyclonal Antibody [FITC]	Rabbit	Human	FACS	FITC
EXA-0822-X240	Rabbit Anti-Mouse CD63 Polyclonal Antibody [FITC]	Rabbit	Mouse	FACS	FITC
EXA-0822-X241	Rabbit Anti-Human CD81 Polyclonal Antibody [FITC]	Rabbit	Human	FACS	FITC
EXA-0822-X242	Rabbit Anti-Human, Mouse, Rat Alix Antibody	Rabbit	Human, Mouse, Rat	WB; IF
EXA-0822-X243	Rabbit Anti-Human, Mouse, Rat Alix Polyclonal Antibody [Conjugated]	Rabbit	Human, Mouse, Rat	WB; IF

For Research Use Only. Cannot be used by patients.