Isolation of exosomal RNA from cell culture media and body fluids is presently a tedious, non-specific, and difficult process. Empowered by leading technologies as well as years of experience in the exosome research, Creative Biolabs has successfully launched an innovative exosomal RNA isolation and profiling platform with various RNA extraction service, including total RNA, mRNA and small RNA.

Background

Depending on the cell/tissue of origin, many different roles and functions have been attributed to exosomes. Recent studies have demonstrated that exosomes are not only specifically targeted to recipient cells to exchange proteins and lipids or to trigger downstream signaling events, but also to deliver specific nucleic acid cargo for cell communication purposes. Exosomal vesicles contain abundant miRNAs, lncRNAs and circRNAs, which are the latest research hotspots in the RNA field. Therefore, it is crucial for us to further understand exosomes to develop reagents, tools and protocols for the isolation, identification and analysis of their RNA content.

Exosomes: biogenesis, composition, and function. Fig.1 Exosomes: biogenesis, composition, and function. (Soria, 2017)

Exosomal RNA Isolation and qPCR Profiling Services

Exosomes derive from monocytic leukemia cell line, umbilical vein endothelial cells, dendritic cells and embryonic stem cell-derived mesenchymal stroma cells, as well as serum, saliva, placenta and breast milk. Creative Biolabs offers professional technologies to isolate exosomes and exosomal extract RNA from various samples. In different studies describing exosomal RNA, a number of alternative RNA extraction methods have been used including phenol based techniques, combined phenol and column based approaches and pure column based technique.

For isolated exosomal RNA, preliminary characterization is essential, so we also provide quantitative reverse transcription-polymerase chain reaction (qRT-PCR) services. The service content and main process are as follows:

Exosomal RNA Isolation and qPCR Analysis

Features of Our Services

  • Choosing the best isolation kit: the RNA obtained by different isolation kits was tested by qPCR for different genes, and the kit with the highest extraction efficiency was selected;
  • Choosing the best reverse transcription kit: the reverse transcription efficiency of different reverse transcription kits at different starting concentrations was compared, and the kit with the highest reverse transcription efficiency was selected;
  • Abundant primer and probe design experience: optimized primer probe detection Ct value is smaller, higher sensitivity, stronger fluorescence signal;
  • Complete verification system: a mature and controllable verification system to verify the source of exosomal RNA vesicles;
  • Rich project experience: exosomal RNA isolation and qPCR verification work involving 10+ sample types has been completed.

With the help of our well-established technologies and experienced scientists, Creative Biolabs is capable of developing exosomal RNA isolation and profiling. With innovative exosome isolation method allowed to recover fully intact exosomes in higher yields, we accept biological sample including plasma, serum, urine and cell culture media. We provide very flexible options for each specific case. We are happy to make it accessible to all kinds of research and industrial customers. Please don’t hesitate to contact us for more information.

Reference

  1. Soria, F.N.; et al. Exosomes, an Unmasked Culprit in Neurodegenerative Diseases. Front Neurosci. 2017, 11:26.
For Research Use Only. Cannot be used by patients.

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