Isolation of exosomal RNA from cell culture media and body fluids is presently a tedious, non-specific, and difficult process. Empowered by leading technologies as well as years of experience in the exosome research, Creative Biolabs has successfully launched an innovative exosomal RNA isolation and profiling platform with various RNA extraction service, including total RNA, mRNA and small RNA.

Exosomes are a type of microvesicle, 30-150 nm diameter in size, received increasing attention over the past decade. Exosomes are secreted by all cell types in culture, and also found naturally in body fluids including blood, saliva, urine, and breast milk. Depending on the cell/tissue of origin, many different roles and functions have been attributed to exosomes. Recent studies have demonstrated that exosomes are not only specifically targeted to recipient cells to exchange proteins and lipids or to trigger downstream signaling events, but also to deliver specific nucleic acid cargo for cell communication purposes.

Exosomal RNA Isolation Methods

Critical to further our understanding of exosomes, is the development of reagents, tools and protocols for their isolation, characterization and analysis of their RNA and protein contents. In different studies describing exosomal RNA, a number of alternative RNA extraction methods have been used including phenol based techniques, combined phenol and column based approaches and pure column based technique. Several reports have been published to date, using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and next generation sequencing for initial characterization of the RNA content of exosomes.

Exosomes: biogenesis, composition, and function.Fig.1 Exosomes: biogenesis, composition, and function. (Soria, 2017)

Exosomal RNA Isolation and Profiling Workflow

Exosomes derive from monocytic leukemia cell line, umbilical vein endothelial cells, dendritic cells and embryonic stem cell-derived mesenchymal stroma cells, as well as serum, saliva, placenta and breast milk. Creative Biolabs offers professional technologies to isolate exosomes and exosomal extract RNA from various samples. The general workflow for exosomal RNA isolation and profiling is outlined below.

Exosomal RNA Isolation and Profiling

Features of Our Services

  • Direct capture and exosome RNA extraction from human biofluids and cell culture media
  • Small volume of biological sample (plasma) per reaction
  • High yield and purity RNA
  • Various RNA extraction service including total RNA, mRNA and small RNA
  • RNA analysis by Bioanalyzer and RT-PCR

With the help of our well-established technologies and experienced scientists, Creative Biolabs is capable of developing exosomal RNA isolation and profiling. With innovative exosome isolation method allowed to recover fully intact exosomes in higher yields, we accept biological sample including plasma, serum, urine and cell culture media. We provide very flexible options for each specific case. We are happy to make it accessible to all kinds of research and industrial customers. Please don’t hesitate to contact us for more information.

Reference:

  1. Soria, F.N.; et al. Exosomes, an Unmasked Culprit in Neurodegenerative Diseases. Front Neurosci2017, 11:26.

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